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Titolo:
CLONING, MAPPING, EXPRESSION, FUNCTION, AND MUTATION ANALYSES OF THE HUMAN ORTHOLOG OF THE HAMSTER PUTATIVE TUMOR-SUPPRESSOR GENE DOC-1
Autore:
TSUJI T; DUH FM; LATIF F; POPESCU NC; ZIMONJIC DB; MCBRIDE J; MATSUO K; OHYAMA H; TODD R; NAGATA E; TERAKADO N; SASAKI A; MATSUMURA T; LERMAN MI; WONG DTW;
Indirizzi:
HARVARD UNIV,SCH DENT MED,DIV ORAL PATHOL,LAB MOL PATHOL,188 LONGWOODAVE BOSTON MA 02115 HARVARD UNIV,SCH DENT MED,DIV ORAL PATHOL,LAB MOL PATHOL BOSTON MA 02115 SAIC,INTRAMURAL RES SUPPORT PROGRAM FREDERICK MD 00000 NCI,FREDERICK CANC RES & DEV CTR,DIV BASIC SCI,IMMUNOBIOL LAB FREDERICK MD 21702 NCI,DIV BASIC SCI,EXPT CARCINOGENESIS LAB,NIH BETHESDA MD 20892 OKAYAMA UNIV,SCH DENT,DEPT ORAL & MAXILLOFACIAL SURG 2 OKAYAMA 700 JAPAN
Titolo Testata:
The Journal of biological chemistry
fascicolo: 12, volume: 273, anno: 1998,
pagine: 6704 - 6709
SICI:
0021-9258(1998)273:12<6704:CMEFAM>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-SITU HYBRIDIZATION; RETINOIC ACID; LOCALIZATION; EOSINOPHILS; KERATIN-19; RECEPTORS; CANCER; ALPHA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
T. Tsuji et al., "CLONING, MAPPING, EXPRESSION, FUNCTION, AND MUTATION ANALYSES OF THE HUMAN ORTHOLOG OF THE HAMSTER PUTATIVE TUMOR-SUPPRESSOR GENE DOC-1", The Journal of biological chemistry, 273(12), 1998, pp. 6704-6709

Abstract

doc-1 is a putative tumor suppressor gene isolated and identified from the hamster oral cancer model, Here, we report the molecular cloningand the functional characterization of the human ortholog of the hamster doc-l gene, Human doc-l cDNA is 1.6 kilobase pairs in length and encodes for a 115-amino acid polypeptide (12.4 kDa, pi 9.53). Sequence analysis showed 98% identity between human and hamster doc-l protein sequences. DOC-1 is expressed in all normal human tissues examined. In oral keratinocytes, expression of DOC-1 is restricted to normal oral keratinocytes, By immunostaining of normal human mucosa, DOC-1 is detected in both the cytoplasm and nuclei of basal oral keratinocytes; while in suprabasilar cells, it is primarily found in the nuclei, Human oral cancers in vivo did not exhibit immunostaining for DOC-1, Like murine DOC-1, human DOC-1 associates with DNA polymerase alpha/primase andmediates the phosphorylation of the large p180 catalytic subunit, suggesting it may be a potential regulator of DNA replication in the S phase of the cell cycle, Using a human doc-l cosmid as a probe, human doc-l is mapped to chromosome 12q24. We identified four exons in the entire human doc-l gene and determined the intron-exon boundaries. By polymerase chain reaction and direct sequencing, we examined premalignantoral lesion and oral cancer cell lines and found no intragenic mutations.

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Documento generato il 07/08/20 alle ore 06:17:13