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Titolo:
INACTIVATION OF TUMOR-NECROSIS-FACTOR-ALPHA BY PROTEINASES (GINGIPAINS) FROM THE PERIODONTAL PATHOGEN, PORPHYROMONAS-GINGIVALIS - IMPLICATIONS OF IMMUNE EVASION
Autore:
CALKINS CC; PLATT K; POTEMPA J; TRAVIS J;
Indirizzi:
UNIV GEORGIA,DEPT BIOCHEM & MOL BIOL ATHENS GA 30602 UNIV GEORGIA,DEPT BIOCHEM & MOL BIOL ATHENS GA 30602 JAGIELLONIAN UNIV,INST MOL BIOL,DEPT MICROBIOL & IMMUNOL PL-31120 KRAKOW POLAND
Titolo Testata:
The Journal of biological chemistry
fascicolo: 12, volume: 273, anno: 1998,
pagine: 6611 - 6614
SICI:
0021-9258(1998)273:12<6611:IOTBP(>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
VASCULAR-PERMEABILITY ENHANCEMENT; BACTEROIDES-GINGIVALIS; CYSTEINE PROTEINASE; CREVICULAR FLUID; DISEASE; CELLS; PURIFICATION; ACTIVATION; GENERATION; CYTOKINES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
C.C. Calkins et al., "INACTIVATION OF TUMOR-NECROSIS-FACTOR-ALPHA BY PROTEINASES (GINGIPAINS) FROM THE PERIODONTAL PATHOGEN, PORPHYROMONAS-GINGIVALIS - IMPLICATIONS OF IMMUNE EVASION", The Journal of biological chemistry, 273(12), 1998, pp. 6611-6614

Abstract

Porphyromonas gingivalis is one of the major pathogens associated with adult periodontitis, a major chronic inflammatory disease. Potent proteinases elaborated by these bacteria aid directly and indirectly in both the development of the pathophysiology of the disease and in hostdefense evasion. For these reasons they are considered key virulence factors. To investigate whether possible immune evasion mechanisms involve the dysregulation of the host cytokine network, we examined the ability of P. gingivalis cysteine proteinases, including Arg-specific gingipains HRGP and RGP2 and Lys-specific KGP, to degrade the proinflammatory cytokine tumor necrosis factor-alpha (TNF-alpha). All three gingipains rapidly degraded TNF-alpha as exhibited by immunoblot analysis. Moreover, all biological activity was significantly reduced over extended incubation periods with the proteinases tested, whereas the hostneutrophil proteinases were ineffective. These results indicate that the gingipain proteinases elaborated by P. gingivalis are capable of disrupting the cytokine network at the site of infection through the degradation of the proinflammatory cytokine TNF-alpha, suggesting the removal of one of several mediators important to the function of polymorphonuclear leukocytes. Such a mechanism is likely to be utilized by other infective organisms not only for survival but also for growth and proliferation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 07:04:06