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Titolo:
OVERPRODUCTION OF SWEET-POTATO PEROXIDASES IN TRANSGENIC TOBACCO PLANTS
Autore:
HUH GH; YUN BW; LEE HS; JO JK; KWAK SS;
Indirizzi:
KOREA RES INST BIOSCI & BIOTECHNOL,PLANT BIOCHEM RES UNIT,POB 115 TAEJON 305600 SOUTH KOREA KOREA RES INST BIOSCI & BIOTECHNOL,PLANT BIOCHEM RES UNIT TAEJON 305600 SOUTH KOREA KYUNGPOOK NATL UNIV,DEPT ANIM SCI TAEGU 702701 SOUTH KOREA
Titolo Testata:
Phytochemistry
fascicolo: 5, volume: 47, anno: 1998,
pagine: 695 - 700
SICI:
0031-9422(1998)47:5<695:OOSPIT>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Keywords:
IPOMOEA BATATAS; CONVOLVULACEAE; NICOTIANA TABACUM; SOLANACEAE; TRANSGENIC PLANTS; PEROXIDASE; SUPEROXIDE DISMUTASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
16
Recensione:
Indirizzi per estratti:
Citazione:
G.H. Huh et al., "OVERPRODUCTION OF SWEET-POTATO PEROXIDASES IN TRANSGENIC TOBACCO PLANTS", Phytochemistry, 47(5), 1998, pp. 695-700

Abstract

Two peroxidase (POD) cDNAs, anionic (swpa1) and neutral (swpn1) POD derived from suspension cultures of sweet potato (Ipomoea batatas), were used to express high levels in transgenic plants. Two sweet potato PODs were overproduced in two cultivars of transformed tobacco (Nicotiana tabacum) plants, cv. Bel W3 and cv. Samsun by introducing a chimeric gene composed of the cauliflower mosaic virus 35S promoter and POD cDNA. Young fully expanded leaves of transgenic plants of both cultivars showed higher POD activity than those of nontransgenic plants. Bel W3 transgenic plants carrying anionic POD had total POD activity ca five times higher than control plants. However, no significant differences were observed in superoxide dismutase activity between transgenic and nontransgenic plants. Transgenic Bel W3 and Samsun tobacco plants with either anionic or neutral POD isoenzyme did not show distinctive phenotypes compared with untransformed plants. (C) 1998 Elsevier ScienceLtd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 15:54:07