Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
SPERM-ASSOCIATED OOCYTE-ACTIVATING FACTOR IS RELEASED FROM THE SPERMATOZOON WITHIN 30 MINUTES AFTER INJECTION AS A RESULT OF THE SPERM-OOCYTE INTERACTION
Autore:
DOZORTSEV D; QIAN C; ERMILOV A; RYBOUCHKIN A; DESUTTER P; DHONT M;
Indirizzi:
WAYNE STATE UNIV,HUTZEL HOSP,DEPT OBSTET GYNECOL,4707 ST ANTOINE BLD DETROIT MI 48201 WAYNE STATE UNIV,DEPT OBSTET & GYNECOL,DIV REPROD ENDOCRINOL & INFERTIL DETROIT MI 00000 STATE UNIV GHENT,DEPT OBSTET & GYNECOL B-9000 GHENT BELGIUM
Titolo Testata:
Human reproduction
fascicolo: 12, volume: 12, anno: 1997,
pagine: 2792 - 2796
SICI:
0268-1161(1997)12:12<2792:SOFIRF>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
CALCIUM OSCILLATIONS; MAMMALIAN EGGS; MOUSE OOCYTES; FERTILIZATION; DECONDENSATION; CELL;
Keywords:
HUMAN; ICSI; MOUSE; SPERM-OOCYTE INTERACTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
D. Dozortsev et al., "SPERM-ASSOCIATED OOCYTE-ACTIVATING FACTOR IS RELEASED FROM THE SPERMATOZOON WITHIN 30 MINUTES AFTER INJECTION AS A RESULT OF THE SPERM-OOCYTE INTERACTION", Human reproduction, 12(12), 1997, pp. 2792-2796

Abstract

We have previously shown that sperm plasma membrane damage makes the sperm plasma membrane permeable and the sperm nucleus accessible for low molecular weight molecules such as eosin and dithiothreitol. In thepresent study, we investigated whether this damage is associated witha passive release of the sperm-associated oocyte activating factor (SAOAF) from the spermatozoon and, if so, its time sequence, In a first study, human oocytes remaining unfertilized after conventional in-vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) and freshly ovulated mouse oocytes were injected with a whole spermatozoon or a sperm head respectively, They were randomly allocated to one of three groups: oocytes in group 1 were injected with a spermatozoon immobilized or sperm head detached immediately prior to the injection; oocytes in group 2 were injected with a spermatozoon immobilized or spermhead detached 2-4 h before injection; oocytes in group 3 were injected with a spermatozoon or sperm head that had been subjected to heat treatment, The activation rate of oocytes injected with a spermatozoon or sperm head was the same for groups 1 and 2, and significantly higherthan in group 3 (P < 0.001), In a second series of experiments, humanoocytes remaining unfertilized after IVF or ICSI were injected with asperm head that was subsequently removed from the ooplasm 20-30 min after injection, The activation rates were compared to that of oocytes injected with heat-treated spermatozoa which subsequently were removedfrom the ooplasm, We found that the removal of the spermatozoon 30 min after injection did not prevent oocyte activation, Our data indicatethat the initial damage to the sperm plasma membrane induced at immobilization, although essential for the onset of sperm nuclear swelling after ICSI, does not by itself lead to the release-of SAOAF from the spermatozoon. We postulate, however, that SAOAF is released during the sperm nuclear swelling phase, which is induced by the so-called sperm nucleus decondensing factor (SNDF) of the oocyte.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 00:57:14