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Titolo:
A RAPID AND SIMPLE METHOD FOR CRYOPRESERVATION OF HUMAN LIVER SLICES
Autore:
DEKANTER R; OLINGA P; HOF I; DEJAGER M; VERWILLEGEN WA; SLOOFF MJH; KOSTER HJ; MEIJER DKF; GROOTHUIS GMM;
Indirizzi:
GRONINGEN UTRECHT INST DRUG EXPLORAT,DEPT PHARMACOKINET & DRUG DELIVERY,ANT DEUSINGLAAN 1 NL-9713 AV GRONINGEN NETHERLANDS SOLVAY DUPHAR BV,DRUG SAFETY DEPT NL-1380 DA WEESP NETHERLANDS UNIV GRONINGEN HOSP,DEPT SURG,GUIDE,DIV HEPATOBILIARY SURG & LIVER TRANSPLANTAT NL-9713 GZ GRONINGEN NETHERLANDS
Titolo Testata:
Xenobiotica
fascicolo: 3, volume: 28, anno: 1998,
pagine: 225 - 234
SICI:
0049-8254(1998)28:3<225:ARASMF>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
TISSUE-SLICES; HEPATOCYTES; METABOLISM; RAT; 7-ETHOXYCOUMARIN; MICROSOMES; CULTURE; MONKEY; PIG;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
R. Dekanter et al., "A RAPID AND SIMPLE METHOD FOR CRYOPRESERVATION OF HUMAN LIVER SLICES", Xenobiotica, 28(3), 1998, pp. 225-234

Abstract

1. Precision-cut liver slices represent a suitable and convenient in vitro preparation for studying metabolism and toxicity mechanisms of drugs and toxic chemicals. Particularly in the case of human liver slices, cryopreservation would enable more efficient utilization of this scarce and irregularly available tissue. 2. Liver slices from consecutive human livers were cryopreserved using a method previously developedfor rat and monkey liver slices. This procedure involves incubation in 12% dimethyl sulphoxide for 30 mir. on ice and direct immersion intoliquid nitrogen. 3. Functional integrity of cryopreserved human liverslices, as compared with that of fresh liver slices, was maintained at 66+/-8% (alanine aminotransferase activity retained in the slices), 78+/-7% (urea synthesis), 88+/-14% (testosterone hydroxylation), 84+/-7% (N-deethylation of lidocaine) and 88+/-10% (total O-deethylation of7-ethoxycoumarin). The ratios of testosterone metabolites did not change on cryopreservation. 4. These results show that the cryopreserved human liver slices retained the measured drug metabolism activities. Therefore, this cryopreservation method is suitable for storing liver slices to be used for comparing drug metabolism patterns, at least qualitatively, between species.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 13:17:20