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Titolo:
IGF-1, PDGF AND CD18 ARE ADHERENCE-RESPONSIVE GENES - REGULATION DURING MONOCYTE DIFFERENTIATION
Autore:
JENDRASCHAK E; KAMINSKI WE; KIEFL R; VONSCHACKY C;
Indirizzi:
UNIV MUNICH,KLINIKUM INNENSTADT,MED KLIN D-80336 MUNICH GERMANY UNIV MUNICH,KLINIKUM INNENSTADT,MED KLIN D-80336 MUNICH GERMANY UNIV WASHINGTON,SCH MED,DEPT PATHOL SEATTLE WA 98195
Titolo Testata:
Biochimica et biophysica acta, N. Gene structure and expression
fascicolo: 3, volume: 1396, anno: 1998,
pagine: 320 - 335
SICI:
0167-4781(1998)1396:3<320:IPACAA>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN MONONUCLEAR-CELLS; VASCULAR ENDOTHELIAL-CELLS; FACTOR MESSENGER-RNA; FACTOR A-CHAIN; GROWTH-FACTOR; TRANSCRIPTION FACTORS; PROMOTER REGION; C-FOS; IDENTIFICATION; EXPRESSION;
Keywords:
GROWTH FACTOR; MONOCYTE/MACROPHAGE; ADHERENCE; MESSENGER-RNA; PCR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
E. Jendraschak et al., "IGF-1, PDGF AND CD18 ARE ADHERENCE-RESPONSIVE GENES - REGULATION DURING MONOCYTE DIFFERENTIATION", Biochimica et biophysica acta, N. Gene structure and expression, 1396(3), 1998, pp. 320-335

Abstract

Unstimulated mononuclear cells express IGF-1, PDGF-A and PDGF-B mRNA,but not a number of other genes coding for growth factors or cytokines, as we demonstrated previously. The main focus of the present investigation was to compare gene expression of mononuclear cells unstimulated in suspension with gene expression of monocytes stimulated by adherence. mRNA levels of IGF-1-A and -B, PDGF-A, -B, PD-ECGF, basic FGF, acidic FGF, TGF-alpha, TGF-beta 1, and IGF-2 were sought for and quantified with our sensitive RT-PCR method (3n-PCR). The respective mRNAs of basic FGF, acidic FGF, TGF-alpha and IGF-2 were not detected, independent of the culture conditions. In suspension culture, mRNA levels ofIGF-1A and -B, PDGF-A, -B, and CD18 remained unchanged. Monocyte adherence regulated IGF-1A, PDGF-A, and -B mRNA levels. In parallel, mRNA levels of the monocyte adhesion molecule CD18 increased rapidly (4.5-fold). In contrast, independent of the presence of an adherence stimulus, the mRNAs for the cytoskeletal structure protein p-actin and PD-ECGF remained constant, whereas mRNA for growth factors TGF-beta 1 and IGF-1B, respectively, was increased. Thus, monocyte adherence selectively regulates IGF-1, PDGF-A, PDGF-B and CD18 mRNAs (adherence-responsivegenes) in a coordinated manner. This led us to identify two novel consensus elements within their respective functional promoters. Both motifs, an 11 bp purine-rich sequence and a 13 bp pyrimidine-rich segment, respectively, are absent from the genes that were not specifically activated by adherence. The identified elements are potential binding sites for transcription factors that may define a common basis for the regulation of the adherence-responsive genes IGF-1A, PDGF-A, PDGF-B and CD18. (C) 1998 Elsevier Science B.V.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 18:06:55