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Titolo:
CDNA CLONING AND SEQUENCING OF CA2+ CALMODULIN-DEPENDENT PROTEIN-KINASE II-ALPHA SUBUNIT AND ITS MESSENGER-RNA EXPRESSION IN DIISOPROPYL PHOSPHOROFLUORIDATE (DFP)-TREATED HEN CENTRAL-NERVOUS-SYSTEM/
Autore:
GUPTA RP; BING GY; HONG JS; ABOUDONIA MB;
Indirizzi:
DUKE UNIV,MED CTR,DEPT PHARMACOL,POB 3813 DURHAM NC 27710 DUKE UNIV,MED CTR,DEPT PHARMACOL DURHAM NC 27710 NIEHS,LAB ENVIRONM NEUROSCI,NIH RES TRIANGLE PK NC 27709
Titolo Testata:
Molecular and cellular biochemistry
fascicolo: 1-2, volume: 181, anno: 1998,
pagine: 29 - 39
SICI:
0300-8177(1998)181:1-2<29:CCASOC>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
INDUCED DELAYED NEUROTOXICITY; CRESYL PHOSPHATE; CYTOSKELETAL PROTEINS; CALMODULIN KINASE; MOLECULAR-CLONING; SCIATIC-NERVE; BRAIN; PHOSPHORYLATION; BINDING; CHICKEN;
Keywords:
BRAIN; CAM KINASE II; CDNA LIBRARY; DIISOPROPYL PHOSPHOROFLUORIDATE; MESSENGER-RNA EXPRESSION; CHICKEN; OPIDN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
R.P. Gupta et al., "CDNA CLONING AND SEQUENCING OF CA2+ CALMODULIN-DEPENDENT PROTEIN-KINASE II-ALPHA SUBUNIT AND ITS MESSENGER-RNA EXPRESSION IN DIISOPROPYL PHOSPHOROFLUORIDATE (DFP)-TREATED HEN CENTRAL-NERVOUS-SYSTEM/", Molecular and cellular biochemistry, 181(1-2), 1998, pp. 29-39

Abstract

Diisopropyl phosphorofluoridate (DFP) produces delayed neurotoxicity,known as organophosphorus ester-induced delayed neurotoxicity (OPIDN), in hen, human, and other sensitive species. A single dose of DFP (1.7 mg/kg, se.) produces first mild ataxia followed by paralysis in 7-14days in hens. DFP treatment also increases in vitro autophosphorylation of Ca2+ calmodulin-dependent protein kinase II (CaM kinase II) and the phosphorylation of several cytoskeletal proteins in the hen brain. To investigate whether increase in CaM kinase II activity is associated with increased expression of its mRNA, we cloned and sequenced CaM kinase II alpha subunit cDNA, and used it to study CaM kinase II expression in brain regions and spinal cord. Hen CaM kinase II a subunit differs in 7 amino acids from that of rat CaM kinase II Its mRNA occurs predominantly as a 6.7 kb message, which is very close to that of human CaM kinase II alpha subunit. Northern blot analysis showed a transient increase in CaM kinase II alpha subunit mRNA in the cerebellum and spinal cord of DFP-treated chickens. The increase in CaM kinase II mRNA expression is consistent with the previously reported increase in its activity in brain and spinal cord, and its increased expression onlyin cerebellum and spinal cord, which are sensitive to the Wallerian-type degeneration characteristic of OPIDN, suggests the probable role of this enzyme in delayed neurotoxicity.

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Documento generato il 20/09/20 alle ore 10:15:59