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Titolo:
NATURAL-KILLER AND B-LYMPHOID POTENTIAL IN CD34(-CELLS DIFFERENTIATEDIN THE PRESENCE OF VASCULAR ENDOTHELIAL GROWTH-FACTOR() CELLS DERIVEDFROM EMBRYONIC STEM)
Autore:
NAKAYAMA N; FANG IW; ELLIOTT G;
Indirizzi:
AMGEN INC,DEPT CELL BIOL,1840 DEHAVILLAND DR THOUSAND OAKS CA 91320
Titolo Testata:
Blood
fascicolo: 7, volume: 91, anno: 1998,
pagine: 2283 - 2295
SICI:
0006-4971(1998)91:7<2283:NABPIC>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-VITRO GENERATION; T RESPONSES INVITRO; MURINE FETAL LIVER; YOLK-SAC; BETA-CHAIN; C-KIT; HEMATOPOIETIC PROGENITORS; RECOMBINANT INTERLEUKIN-2; REGULATED EXPRESSION; DEVELOPING MOUSE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
86
Recensione:
Indirizzi per estratti:
Citazione:
N. Nakayama et al., "NATURAL-KILLER AND B-LYMPHOID POTENTIAL IN CD34(-CELLS DIFFERENTIATEDIN THE PRESENCE OF VASCULAR ENDOTHELIAL GROWTH-FACTOR() CELLS DERIVEDFROM EMBRYONIC STEM)", Blood, 91(7), 1998, pp. 2283-2295

Abstract

Differentiation of totipotent mouse embryonic stem (ES) cells to various lymphohematopoietic cells is an in vitro model of the hematopoietic cell development during embryogenesis. To understand this process atcellular levels, differentiation intermediates were investigated. ES cells generated progeny expressing CD34, which was significantly enhanced by vascular endothelial growth factor (VEGF). The isolated CD34(+)cells were enriched for myeloid colony-forming cells but not significantly for erythroid colony-forming cells. When cultured on OP9 stroma cells in the presence of interleukin-2 and interleukin-7, the CD34(+) cells developed two types of B220(+) CD34(-) lymphocytes: CD3(-) cytotoxic lymphocytes and CD19(+) preB cells, and such lymphoid potential was highly enriched in the CD34(+) population. Interestingly. the cytotoxic cells expressed the natural killer (NK) cell markers. such as NKR-P1. perforin. and granzymes, classified into two types, one of which showed target specificity of NK cells. Thus, ES cells have potential to generate NK-type cytotoxic lymphocytes in vitro in addition to erythro-myeloid cells and pre-B cells, and both myeloid and lymphoid cells seem to be derived from the CD34(+) intermediate, on which VEGF may play an important role. (C) 1998 by The American Society of Hematology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/07/20 alle ore 09:02:19