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Titolo:
MURINE BONE-MARROW STROMALLY DERIVED BMS2 ADIPOCYTES SUPPORT DIFFERENTIATION AND FUNCTION OF OSTEOCLAST-LIKE CELLS IN-VITRO
Autore:
KELLY KA; TANAKA S; BARON R; GIMBLE JM;
Indirizzi:
UNIV OKLAHOMA,HLTH SCI CTR,DEPT SURG,POB 26901 OKLAHOMA CITY OK 73190 UNIV OKLAHOMA,HLTH SCI CTR,DEPT PATHOL OKLAHOMA CITY OK 73109 UNIV OKLAHOMA,HLTH SCI CTR,DEPT SURG OKLAHOMA CITY OK 73109 UNIV OKLAHOMA,HLTH SCI CTR,DEPT ORTHODONT OKLAHOMA CITY OK 73109 OKLAHOMA MED RES FDN OKLAHOMA CITY OK 73104 UNIV TOKYO,DEPT ORTHOPED SURG TOKYO 113 JAPAN YALE UNIV,SCH MED,DEPT ORTHOPED & CELL BIOL NEW HAVEN CT 06510
Titolo Testata:
Endocrinology
fascicolo: 4, volume: 139, anno: 1998,
pagine: 2092 - 2101
SICI:
0013-7227(1998)139:4<2092:MBSDBA>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; LYMPHOCYTE GROWTH; PROLIFERATION; MOUSE; EXPRESSION; HEMATOPOIESIS; MC3T3-G2/PA6; CULTURES; RECEPTOR; LINES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
51
Recensione:
Indirizzi per estratti:
Citazione:
K.A. Kelly et al., "MURINE BONE-MARROW STROMALLY DERIVED BMS2 ADIPOCYTES SUPPORT DIFFERENTIATION AND FUNCTION OF OSTEOCLAST-LIKE CELLS IN-VITRO", Endocrinology, 139(4), 1998, pp. 2092-2101

Abstract

Stromal cells are required for in vitro osteoclast differentiation and maturation. The murine bone marrow stromally derived BMS2 calf line exhibits adipocytic and osteoblastic features as well as the ability to support lymphopoiesis and myelopoiesis. This work examined the ability of the BMS2 cell in either the preadipocyte or adipocyte state to support the formation of osteoclast-like cells. BMS2 cells can be induced to undergo adipogenic differentiation in response to treatment withglucocorticoids or thiazolidinedione compounds. Primary bone marrow cells, enriched for hematopoietic progenitors and depleted of their adherent stromal and macrophage populations, were stimulated with vitaminD-3 (vitamin D; 10(-8) M) to undergo osteoclast differentiation and maturation when cocultured with BMS2 cells. In both. preadipocyte and adipocyte-enriched BMS2 stromal layers, comparable numbers of tartrate-resistant acid phosphatase-posit osteoclast-like cells, characterized by their response to salmon calcitonin with an increase in cAMP and formation of resorption pits on bovine bone slices, were formed. The gene expression and protein levels of macrophage colony-stimulating factor produced by preadipocyte and adipocyte-rich BMS2 layers were comparable. However, adipocyte-rich stromal layers supported osteoclast-like cell formation longer in culture than preadipocytes, independent of the agent used to induce adipocyte differentiation. These studies demonstrate for the first time that fully differentiated adipocyte stromal cells can support osteoclast-like cell formation and function in vitro.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 13:11:11