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Titolo:
HOMOLOGOUS AND HETEROLOGOUS ACUTE DESENSITIZATION OF VASOPRESSIN V1A RECEPTOR IN XENOPUS OOCYTES
Autore:
ANCELLIN N; MOREL A;
Indirizzi:
UNIV ANGERS,2 RUE LAVOISIER F-49000 ANGERS FRANCE UNIV ANGERS F-49000 ANGERS FRANCE CEA SACLAY,DEPT BIOL CELLULAIRE & MOL,SERV BIOL CELLULAIRE F-91191 GIF SUR YVETTE FRANCE
Titolo Testata:
Cellular signalling
fascicolo: 3, volume: 10, anno: 1998,
pagine: 217 - 223
SICI:
0898-6568(1998)10:3<217:HAHADO>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEPENDENT PROTEIN-KINASE; ARGININE-VASOPRESSIN; MECHANISMS; POTENT; CELLS;
Keywords:
V1A VASOPRESSIN RECEPTOR; M5 MUSCARINIC RECEPTOR; DESENSITIZATION; DAG ANALOGS; PHORHOL ESTERS; PKC; AEQUORIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
N. Ancellin e A. Morel, "HOMOLOGOUS AND HETEROLOGOUS ACUTE DESENSITIZATION OF VASOPRESSIN V1A RECEPTOR IN XENOPUS OOCYTES", Cellular signalling, 10(3), 1998, pp. 217-223

Abstract

The mechanism of short-term desensitisation of the V1a vasopressin receptor, a phospholipase-C beta linked receptor, was investigated in albino Xenopus oocytes. V1a receptors showed rapid agonist-dependent mobilisation of intracellular calcium, as detected by aequorin photon emission. Agonist-induced homologous short-term desensitisation was evidenced within minutes after stimulation. Injection of the second messengers calcium or inositol triphosphate inside the cell did not desensitise the receptors. In contrast, protein kinase C (PKC) activators 1-oleoyl-2-acetyl-sn-glycerol (OAG) (50 mu M) and 1,2-dioctanoyl-glycerol (DIC8) (10 mu M), as well as phorbol-12-myristate-13-acetate (1 mu M) and phorbol-12,13-dibutyrate (1 mu M) blunted the calcium responsiveness of the V1a receptors. The specific PKC inhibitor bisindolylmaleimide(GF109203X) (1 mu M) prevented the effect of DIC8 and OAG on V1a receptor desensitisation. Heterologous desensitisation induced by agonist occurred in oocytes that co-expressed the V1a receptor and the PKC-activating M5 muscarinic receptor. It was concluded that PKC activation has a role in short-term desensitisation of the V1a receptor. (C) 1998 Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/04/20 alle ore 02:25:50