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Titolo:
POTENTIATION OF CHEMOTACTIC PEPTIDE-INDUCED SUPEROXIDE GENERATION BY CD38 LIGATION IN HUMAN MYELOID CELL-LINES
Autore:
TSUJIMOTO N; KONTANI K; INOUE S; HOSHINO S; HAZEKI O; MALAVASI F; KATADA T;
Indirizzi:
UNIV TOKYO,FAC PHARMACEUT SCI,DEPT PHYSIOL CHEM,BUNKYO KU TOKYO 113 JAPAN UNIV TOKYO,FAC PHARMACEUT SCI,DEPT PHYSIOL CHEM,BUNKYO KU TOKYO 113 JAPAN UNIV ANCONA,SCH MED,INST BIOL & GENET ANCONA ITALY
Titolo Testata:
Journal of Biochemistry
fascicolo: 5, volume: 121, anno: 1997,
pagine: 949 - 956
SICI:
0021-924X(1997)121:5<949:POCPSG>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYCLIC ADP-RIBOSE; LEUKEMIC HL-60 CELLS; SURFACE ANTIGEN CD38; TYROSINE PHOSPHORYLATION; NAD GLYCOHYDROLASE; RETINOIC ACID; EXPRESSION; ACTIVATION; MOLECULE; LYMPHOCYTES;
Keywords:
CD38; CHEMOTACTIC RECEPTORS; G PROTEINS; TYROSINE PHOSPHORYLATION; SUPEROXIDE GENERATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
N. Tsujimoto et al., "POTENTIATION OF CHEMOTACTIC PEPTIDE-INDUCED SUPEROXIDE GENERATION BY CD38 LIGATION IN HUMAN MYELOID CELL-LINES", Journal of Biochemistry, 121(5), 1997, pp. 949-956

Abstract

CD38 is a type II transmembrane glycoprotein possessing an NAD(+) glycohydrolase activity in its extracellular domain, We previously reported that the ligation of CD38 by a monoclonal antibody (mAb), HE-7, induces the tyrosine phosphorylation of cellular proteins including p120(c-cbl) in differentiated human myeloid cell lines and that the phosphorylated p120(c-cbl) is capable of binding to phosphatidylinositol (PI)3-kinase. In the present study, we found that the agonistic anti-CD38mAb markedly potentiates superoxide generation stimulated by chemotactic formyl-Met-Leu-Phe receptors in the CD38-producing cells, HE-7 neither generated superoxide by itself nor enhanced the cell response induced by phorbol 12-myristate acetate, indicating that the potentiatingaction of the anti-CD38 mAb is specific for the stimulation by the GTP-binding protein (G(1))-coupled membrane receptors, The potentiation by HE-7 was abolished by prior treatment of the cells with a tyrosine kinase inhibitor, pertussis toxin, or a potent PI 3-kinase inhibitor, wortmannin. HE-7 also enhanced the product formation of PI 3-kinase inresponse to the chemotactic receptor stimulation, without significantchanges in the receptor-stimulated accumulations of inositol-1,4,5-trisphosphate, arachidonate release, and intracellular Ca2+. These results indicate that the CD38-induced tyrosine phosphorylation has a crosstalk with the chemotactic receptor/G(i)-mediated signal transduction pathway resulting in the enhancement of superoxide generation, probablythrough the activation of PI 3-kinase.

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Documento generato il 04/12/20 alle ore 19:56:26