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Titolo:
LIGHT-MICROSCOPIC EXAMINATION OF CYTOMORP HOLOGY AND CYTOGENETICS IN NON-FERTILIZED OOCYTES AFTER IN-VITRO FERTILIZATION AND INTRACYTOPLASMATIC SPERM INJECTION
Autore:
SCHILL T; ALHASANI S; KUPKER W; DIEDRICH K;
Indirizzi:
UNIV LUBECK,KLIN FRAUENHEILKUNDE & GEBURTSHILFE,RATZEBURGER ALLEE 160D-23538 LUBECK GERMANY
Titolo Testata:
Geburtshilfe und Frauenheilkunde
fascicolo: 2, volume: 58, anno: 1998,
pagine: 79 - 87
SICI:
0016-5751(1998)58:2<79:LEOCHA>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
GER
Soggetto:
PREMATURE CHROMOSOME CONDENSATION; METAPHASE-II ARREST; MOUSE OOCYTES; ASSISTED FERTILIZATION; INTERPHASE NUCLEI; IVF PROGRAM; EARLY LIFE; MATURATION; ACTIVATION; EGGS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
63
Recensione:
Indirizzi per estratti:
Citazione:
T. Schill et al., "LIGHT-MICROSCOPIC EXAMINATION OF CYTOMORP HOLOGY AND CYTOGENETICS IN NON-FERTILIZED OOCYTES AFTER IN-VITRO FERTILIZATION AND INTRACYTOPLASMATIC SPERM INJECTION", Geburtshilfe und Frauenheilkunde, 58(2), 1998, pp. 79-87

Abstract

Purpose: In up to 50% of all cases, male infertility is the main reason why a couple's wish to have children remains unfulfilled. The fertilisation rate of 52% achieved by IVF could be increased by the furtherdevelopment of ICSI to values of approx. 70%. A frequent cause for failure of fertilization after classical IVF was that the spermatozoa did not penetrate into the oocyte; this changed as ICSI and the secure injection of the spermatozoa into the oocyte. Why the fertilization rate could not be improved further remains to be investigated by many studies. In case of normal fertilization and also in case of IVF, the spermatozoon fuses with oocyte membrane, is incorporated and the. spermatozoon nucleus enters the cytoplasm without membrane. Consequently cytoplasmatic factors react immediately with the spermatozoon. After ICSI,not a ''naked spermatozoa'' becomes injected but the entire spermatozoon with membrane. This can be cause for a different reaction comparedto a spermatozoon incorporated normally. Purpose of this study is to assess the cytogenetic and cytological reasons for unfertilised oocytes. Material and Method: The cytomorphological and cytogenetical study evaluated 105 unfertilised oocytes of 39 patients. Fixation and staining of the oocytes was done according to a modificated method of Tarkowski. Results: 55.2% (n=58) oocytes were evaluable. The main issue was to examine the spermatozoa which were represented in 45 oocytes. The sperms were mainly (n=36) located in the middle of the oocyte, seven atthe oocyte's edge, one seemed likely in the perivitelline space and one sperm within the chromosomes. The sperms were condensed in 28.2% (n=13). Prematurely condensed sperms (PCC I-III) were found in 71.8% (n=32). We could subdivide them as follows: type I: 60% (n=27), type II: 8.8% (n=4) and type III: 2.2% (n=1). In 56 oocytes chromosomes could be represented. 51.8% were in the haploid field and the remaining part distributes itself over hypoploidy, fragmented, clotted and fluffy chromosomes. Conclusion: Condensed chromosomes as well as prematurely condensed chromosomes are the main factors for non-fertilisation of the oocyte by the sperm. The cause for the PCC is failure of oocyte activation as well as the absence of a spermatozoon-associated oocyte activating factor. Chromosomal abnormalities as a reason for non-fertilisation of the oocyte were found in 48.2%.

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Documento generato il 25/11/20 alle ore 08:46:42