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Titolo:
BOTH NA-K+ ATPASE AND NA+-H+ EXCHANGER ARE IMMEDIATELY ACTIVE UPON POST ISCHEMIC REPERFUSION IN ISOLATED RAT HEARTS()
Autore:
VANEMOUS JG; SCHREUR JHM; RUIGROK TJC; VANECHTELD CJA;
Indirizzi:
UNIV UTRECHT HOSP,HEART LUNG INST,DEPT CARDIOL,ROOM G02-523,POB 85500NL-3508 GA UTRECHT NETHERLANDS UNIV UTRECHT HOSP,HEART LUNG INST,DEPT CARDIOL NL-3508 GA UTRECHT NETHERLANDS UNIV UTRECHT HOSP,INTERUNIV CARDIOL INST NETHERLANDS NL-3508 GA UTRECHT NETHERLANDS
Titolo Testata:
Journal of Molecular and Cellular Cardiology
fascicolo: 2, volume: 30, anno: 1998,
pagine: 337 - 348
SICI:
0022-2828(1998)30:2<337:BNAANE>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEAR-MAGNETIC-RESONANCE; INTRACELLULAR SODIUM RISE; ACUTE MYOCARDIAL-ISCHEMIA; CARDIAC PURKINJE-FIBERS; GLOBAL-ISCHEMIA; POSTISCHEMIC RECOVERY; VENTRICULAR MYOCYTES; SARCOLEMMAL ENZYMES; CALCIUM EXCHANGE; CA-2+ OVERLOAD;
Keywords:
INTRACELLULAR SODIUM; POSTISCHEMIC REPERFUSION; SODIUM-POTASSIUM ATPASE; SODIUM-PROTON EXCHANGE; NA-23-NMR SPECTROSCOPY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
56
Recensione:
Indirizzi per estratti:
Citazione:
J.G. Vanemous et al., "BOTH NA-K+ ATPASE AND NA+-H+ EXCHANGER ARE IMMEDIATELY ACTIVE UPON POST ISCHEMIC REPERFUSION IN ISOLATED RAT HEARTS()", Journal of Molecular and Cellular Cardiology, 30(2), 1998, pp. 337-348

Abstract

Limited time resolution has hampered proper evaluation of changes in intracellular Na+ (Na-i(+)) in whole hearts upon post-ischemic reperfusion. In isolated rat hearts perfused at 37 degrees C, we studied the contribution of the Na+-K+ ATPase and the Na+-H+ exchanger to control of Na+, during reperfusion using Na-23 NMR and the shift reagent Tm(DOTP)(5-) with a time resolution of 5 s. To assess activities of the Na+-K+ ATPase and the Na+-H+ exchanger, 250 mu mol/l ouabain and/or 3 mu mol/l EIPA, respectively, was added to the perfusate during the first 5 min of reperfusion, following 20 min of ischemia. When used, ouabainwas also present for 2 min prior to ischemia. Na-i(+) increased during ouabain perfusion prior to ischemia (132 +/- 5 and 133 +/- 4% of thepre-ischemic control value after 2 min, in ouabain and ouabain + EIPAhearts, respectively; mean +/- S.E.M,; n = 6 per group) resulting in higher end-ischemic Values in ouabain and ouabain + EIPA hearts (249 +/- 9 and 267 +/- 17% of the pre-ischemic control value, respectively) than in control and EIPA hearts (207 +/- 21 and 199 +/- 10% of the pre-ischemic control value, respectively). In ouabain, hearts Na-i(+) started to rise directly upon reperfusion and amounted to 117 +/- 6% of the end-ischemic value after 60 s of reperfusion. In control hearts, however, Na-i(+), dropped immediately and was 87 +/- 5% of the end-ischemic value after 60 s, indicating that the Na+-K+ ATPase resumed function directly upon reperfusion. The initial steep increase of Na-i(+) upon reperfusion in ouabain hearts, which diminished after similar to 40s to the rate of increase observed during ischemia, was absent in ouabain + EIPA hearts. This indicates the existence, although masked by Na+-K+ ATPase activity, of a Na+-H+ exchange mediated Na+ influx upon reperfusion, If only EIPA was present during reperfusion the initial decrease in Na-i(+) was faster than in control hearts, corroborating this finding. (C) 1998 Academic Press Limited.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 18:43:52