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Titolo:
DIHYDROPYRIDINE RECEPTOR GENE-EXPRESSION IN SKELETAL-MUSCLE FROM MDX AND CONTROL MICE
Autore:
PEREON Y; DETTBARN C; NAVARRO J; NOIREAUD J; PALADE PT;
Indirizzi:
UNIV TEXAS,MED BRANCH,DEPT PHYSIOL & BIOPHYS GALVESTON TX 77555 UNIV TEXAS,MED BRANCH,DEPT PHYSIOL & BIOPHYS GALVESTON TX 77555 HOP HOTEL DIEU,INSERM CJF 96 01 NANTES FRANCE
Titolo Testata:
Biochimica et biophysica acta. Molecular basis of disease
fascicolo: 2-3, volume: 1362, anno: 1997,
pagine: 201 - 207
SICI:
0925-4439(1997)1362:2-3<201:DRGISF>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
DUCHENNE MUSCULAR-DYSTROPHY; ELEVATED FREE CALCIUM; TWITCH MUSCLE; CHANNEL; MOUSE; DIAPHRAGM; FIBERS; CURRENTS; HAMSTER; RAT;
Keywords:
EXCITATION-CONTRACTION COUPLING; DIHYDROPYRIDINE RECEPTOR; SKELETAL MUSCLE; DIAPHRAGM; [H-3]-PN200-110 BINDING; MDX; MUSCULAR DYSTROPHY;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
Y. Pereon et al., "DIHYDROPYRIDINE RECEPTOR GENE-EXPRESSION IN SKELETAL-MUSCLE FROM MDX AND CONTROL MICE", Biochimica et biophysica acta. Molecular basis of disease, 1362(2-3), 1997, pp. 201-207

Abstract

The expression of isoform-specific dihydropyrine receptor-calcium channel (DHPR) alpha 1-subunit genes was investigated in mdx and control mouse diaphragm (DIA) and tibialis anterior (TA). RNase protection assays were carried out with a rat DHPR cDNA probe specific for skeletal muscle and a mouse DHPR cDNA probe specific for cardiac muscle. The level of expression of the gene encoding the cardiac DHPR was very weak in TA muscle from both control and mdx mice. Compared to TA, DLA. expressed mRNA for the cardiac isoform at significantly higher levels, butmdx and control mouse DIA levels were similar to one another. In contrast, mRNA expression levels for the DHPR skeletal muscle isoform werelower in control DIA than TA. However, there was a dramatic increase in the expression for the DHPR skeletal muscle isoform in mdr DIA compared with control DIA, reaching the TA expression level, whereas dystrophy did not affect TA expression. [H-3]-PN200-110 binding was used tofurther assess DIA DHPR expression at the protein level. The density of binding sites for the probe was not significantly affected in DIA muscles of mdr vs. control mice, but it was reduced in older mdx and control mice. The increase in DHPR mRNA levels without a consequent increase in DHPR protein expression could be secondary to possible enhanced protein degradation which occurs in mdx DIA. The altered DHPR expression levels found here do not appear to be responsible for the severe deficits in contractile function of the mdx DIA. (C) 1997 Elsevier Science B.V.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 20:56:55