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Titolo:
A MAIZE ANTHOCYANIN TRANSACTIVATOR INDUCES PIGMENTATION IN HAIRY ROOTS OF DICOTYLEDONOUS SPECIES
Autore:
DAMIANI F; PAOLOCCI F; CONSONNI G; CREA F; TONELLI C; ARCIONI S;
Indirizzi:
CNR,IST RIC MIGLIORAMENTO GENET PIANTE FORAGGERE,VIA MADONNA ALTE 130I-06128 PERUGIA ITALY UNIV MILAN,DIPARTIMENTO AGRON I-20122 MILAN ITALY UNIV MILAN,DIPARTIMENTO GENET MICROORGANISMI I-20122 MILAN ITALY
Titolo Testata:
Plant cell reports
fascicolo: 5, volume: 17, anno: 1998,
pagine: 339 - 344
SICI:
0721-7714(1998)17:5<339:AMATIP>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
AGROBACTERIUM-RHIZOGENES 1855; R-GENE FAMILY; REGULATORY GENE; TRANSCRIPTIONAL ACTIVATORS; NUCLEOTIDE-SEQUENCE; MOLECULAR ANALYSIS; GROWTH-REGULATORS; VISIBLE MARKER; PLASMID; HOMOLOGY;
Keywords:
GENETIC TRANSFORMATION; REPORTER GENE; AGROBACTERIUM RHIZOGENES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
F. Damiani et al., "A MAIZE ANTHOCYANIN TRANSACTIVATOR INDUCES PIGMENTATION IN HAIRY ROOTS OF DICOTYLEDONOUS SPECIES", Plant cell reports, 17(5), 1998, pp. 339-344

Abstract

Several dicotyledonous species were infected with an Agrobacterium rhizogenes binary vector harbouring the plasmid 121.Sn which contains the maize gene Sn under the constitutive promoter CaMV35S. In maize, Sn transactivates the anthocyanin pathway in different tissues. The aim of this work was to test the efficiency of this gene to regulate the anthocyanin pathway in heterologous systems and verify its suitability as a reporter gene. The pigmentation of the hairy roots was compared with hairy roots stained for beta-glucuronidase activity, which were used as a control. In two polymorphic genotypes of Lotus angustissimus, DNA integration and expression were assayed. The maize gene is competent to induce anthocyanin pigmentation in different species, but the complexity of the regulatory mechanisms of anthocyanin synthesis restricts the use of Sn as a reporter gene.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/21 alle ore 15:52:01