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Titolo:
VIROLOGICAL AND MOLECULAR DEMONSTRATION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-2 VERTICAL TRANSMISSION
Autore:
CAVACOSILVA P; TAVEIRA NC; ROSADO L; LOURENCO MH; MONIZPEREIRA J; DOUGLAS NW; DANIELS RS; SANTOSFERREIRA MO;
Indirizzi:
UNIV LISBON,FAC FARM,DEPT MICROBIOL,AV FORCAS ARMADAS P-1600 LISBON PORTUGAL HOSP DONA ESTEFANIA P-1150 LISBON PORTUGAL NATL INST MED RES LONDON NW7 1AA ENGLAND
Titolo Testata:
Journal of virology
fascicolo: 4, volume: 72, anno: 1998,
pagine: 3418 - 3422
SICI:
0022-538X(1998)72:4<3418:VAMDOH>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
TO-CHILD TRANSMISSION; VIRAL PHENOTYPE; HIV-1 INFECTION; COTE-DIVOIRE; AIDS; RETROVIRUS; CAPACITY; VARIANTS; CULTURE; ABIDJAN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
P. Cavacosilva et al., "VIROLOGICAL AND MOLECULAR DEMONSTRATION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-2 VERTICAL TRANSMISSION", Journal of virology, 72(4), 1998, pp. 3418-3422

Abstract

To demonstrate that human immunodeficiency virus type 2 (HIV-2) mother-to child transmission exists, HIV-2 isolates were obtained from bothan asymptomatic mother (HIV-2 strain ARM) and her child (HIV-2 strainSAR), who had a diagnosis of AIDS. To determine their biological phenotype, primary isolates were used to infect various primary mononuclear cells and cell lines, HIV-2 ARM replicates in primary cells and Jurkat- tat, while HIV-2 SAR infects these cells plus SupT1, which led us to classify HIV-2 ARM as a slow/low virus and HIV-2 SAR as having an intermediate (slow/low-3) phenotype. Molecular analysis of the env region corresponding to gp125 was performed. Viral DNA was cloned, sequenced, and used to construct phylogenetic trees. The DNA sequence analysis demonstrated an overall nucleotide diversity of 7.6%. The results present evidence that the child's strain is more virulent than the mother's strain, which is in agreement with the immunodeficiency of the child. The phylogenetic trees that were constructed demonstrate that the two isolates cluster together, being closer to each other than to any other isolate described until now.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 06:19:54