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Titolo:
EFFECTS OF HUMAN TYPE 1-ALPHA METABOTROPIC GLUTAMATE-RECEPTOR EXPRESSION LEVEL ON PHOSPHOINOSITIDE AND CA2+ SIGNALING IN AN INDUCIBLE CELL EXPRESSION SYSTEM
Autore:
HERMANS E; YOUNG KW; CHALLISS RAJ; NAHORSKI SR;
Indirizzi:
UNIV LEICESTER,DEPT CELL PHYSIOL & PHARMACOL,MED SCI BLDG,UNIV RD LEICESTER LE1 9HN LEICS ENGLAND
Titolo Testata:
Journal of neurochemistry
fascicolo: 4, volume: 70, anno: 1998,
pagine: 1772 - 1775
SICI:
0022-3042(1998)70:4<1772:EOHT1M>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
MGLUR1-ALPHA; DERIVATIVES; DOMAIN;
Keywords:
TYPE 1-ALPHA METABOTROPIC GLUTAMATE RECEPTOR; PHOSPHOINOSITIDE HYDROLYSIS; CALCIUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
15
Recensione:
Indirizzi per estratti:
Citazione:
E. Hermans et al., "EFFECTS OF HUMAN TYPE 1-ALPHA METABOTROPIC GLUTAMATE-RECEPTOR EXPRESSION LEVEL ON PHOSPHOINOSITIDE AND CA2+ SIGNALING IN AN INDUCIBLE CELL EXPRESSION SYSTEM", Journal of neurochemistry, 70(4), 1998, pp. 1772-1775

Abstract

Stable expression of the human type 1 alpha metabotropic glutamate (mGlu1 alpha) receptor was achieved in Chinese hamster ovary cells usingan isopropyl-beta-D-thiogalactoside (IPTG)-repressible expression system. Treatment of the cells with IPTG resulted in a time-and concentration-dependent induction of receptor expression. Maximal expression was obtained after treatment of the cells with 100 mu M IPTG for 20 h, leading to a marked increase in receptor immunoreactivity detected by western blot, >30-fold stimulation of H-3-labelled inositol phosphate (H-3-InsP) production, and a robust increase in intracellular calcium concentration in single cells after stimulation with 20 mu M quisqualate. The basal level of H-3-InsP accumulation in cells induced with IPTGwas increased by two-to threefold as compared with control cells; however, this basal activity was found to be dependent on glutamate released by the cells into the incubation medium. Following IPTG treatment,stable expression of the mGlu1 alpha receptor was maintained for at least 1 week. Taken together, these results clearly indicate the advantages of working with an inducible expression system when studying the biochemical and pharmacological properties of the human mGlu1 alpha receptor in transfected cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 11:46:18