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Titolo:
PROLONGED GLUCOCORTICOID EXPOSURE DEPHOSPHOUYLATES HISTONE H1 AND INACTIVATES THE MMTV PROMOTER
Autore:
LEE HL; ARCHER TK;
Indirizzi:
UNIV WESTERN ONTARIO,DEPT OBSTET & GYNAECOL,LONDON REG CANC CTR,790 COMMISSIONERS RD E LONDON ON N6A 4L6 CANADA UNIV WESTERN ONTARIO,DEPT OBSTET & GYNAECOL,LONDON REG CANC CTR LONDON ON N6A 4L6 CANADA UNIV WESTERN ONTARIO,LONDON REG CANC CTR,DEPT BIOCHEM LONDON ON N6A 4L6 CANADA UNIV WESTERN ONTARIO,LONDON REG CANC CTR,DEPT ONCOL LONDON ON N6A 4L6CANADA
Titolo Testata:
EMBO journal
fascicolo: 5, volume: 17, anno: 1998,
pagine: 1454 - 1466
SICI:
0261-4189(1998)17:5<1454:PGEDHH>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSCRIPTION FACTOR ACCESS; RNA POLYMERASE-II; ALTER CHROMATIN STRUCTURE; TUMOR VIRUS PROMOTER; IN-VIVO; LINKER HISTONES; NUCLEOSOMAL DNA; FACTOR-BINDING; YEAST; MOUSE;
Keywords:
CHROMATIN REMODELING; DEPHOSPHORYLATION; GLUCOCORTICOIDS; HISTONE H1; MMTV PROMOTER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
71
Recensione:
Indirizzi per estratti:
Citazione:
H.L. Lee e T.K. Archer, "PROLONGED GLUCOCORTICOID EXPOSURE DEPHOSPHOUYLATES HISTONE H1 AND INACTIVATES THE MMTV PROMOTER", EMBO journal, 17(5), 1998, pp. 1454-1466

Abstract

Glucocorticoids rapidly induce transcription from the mouse mammary tumour virus (MMTV) promoter via a glucocorticoid receptor (GR)-mediated chromatin disruption event, This remodelling of chromatin is transient such that upon prolonged exposure to hormone the promoter becomes refractory to glucocorticoids, We demonstrate that this refractory state requires the continual presence of hormone and can be reversed lay its removal. Our experiments show that the promoter is inactivated via a mechanism whereby histone H1 is dephosphorylated in response to glucocorticoids. Removal of glucocorticoids results in the rephosphorylation of histone H1 and the reacquisition of transcriptional competence by the promoter. This response is specific for the MMTV promoter assembled as chromatin and is not observed for another inducible gene or transiently transfected MMTV DNA. Finally, we demonstrate that H1 on the MMTV promoter is dephosphorylated when the promoter is unresponsive toglucocorticoids, These studies indicate that phosphorylated H1 is intimately linked with the GR-mediated disruption of MMTV chromatin in vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 02:55:31