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Titolo:
A PROTEIN IDENTICAL TO THE YOLK PROTEIN IS STORED IN THE TESTIS IN MALE RED-SEA URCHIN, PSEUDOCENTROTUS-DEPRESSUS
Autore:
UNUMA T; SUZUKI T; KUROKAWA T; YAMAMOTO T; AKIYAMA T;
Indirizzi:
NATL RES INST AQUACULTURE,INLAND STN TAMAKI MIE 51904 JAPAN NATL RES INST AQUACULTURE NANSEI MIE 51601 JAPAN
Titolo Testata:
The Biological bulletin
fascicolo: 1, volume: 194, anno: 1998,
pagine: 92 - 97
SICI:
0006-3185(1998)194:1<92:APITTY>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLYCOPROTEIN; VITELLIN; OVARY; VITELLOGENIN; PURIFICATION; CELOMOCYTES; PRECURSOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
17
Recensione:
Indirizzi per estratti:
Citazione:
T. Unuma et al., "A PROTEIN IDENTICAL TO THE YOLK PROTEIN IS STORED IN THE TESTIS IN MALE RED-SEA URCHIN, PSEUDOCENTROTUS-DEPRESSUS", The Biological bulletin, 194(1), 1998, pp. 92-97

Abstract

Female sea urchins store the major yolk protein (MYP) in ovarian nutritive phagocytes before vitellogenesis. Using immunological procedures, we detected MYP in the testicular nutritive phagocytes of Pseudo-centrotus depressus, the red sea urchin, and then compared the distribution of MYP between sexes during gametogenesis. MYP was purified from unfertilized eggs by ion exchange chromatography (Q Sepharose) and gel filtration (Superdex 200), and an antiserum (anti-MYP) was raised against MYP. Immunoblot analysis demonstrated that immature testes, as wellas ovaries, contained a large quantity of MYP. Immunohistochemistry showed that MYP was distributed in the nutritive phagocytes occupying the follicular lumen in both males and females. In both sexes, as gametogenesis proceeded, the nutritive phagocytes degenerated and the gonadal lumen filled with gametes. MYP accumulated in ripe ova as a yolk protein in the mature ovary. In contrast, MYP was not detected in maturetestes, because stored spermatozoa did not react with anti-MYP. We conclude that in male P. depressus, MYP is stored in the testicular nutritive phagocytes and utilized as the nutrient source for spermatogenesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 18:42:27