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Titolo:
THE RDNA INTERNAL TRANSCRIBED SPACER REGION AS A TAXONOMIC MARKER FORNEMATODES
Autore:
POWERS TO; TODD TC; BURNELL AM; MURRAY PCB; FLEMING CC; SZALANSKI AL; ADAMS BA; HARRIS TS;
Indirizzi:
UNIV NEBRASKA,DEPT PLANT PATHOL,406 PLANT SCI HALL LINCOLN NE 68583 KANSAS STATE UNIV,DEPT PLANT PATHOL MANHATTAN KS 66506 ST PATRICKS COLL,DEPT BIOL MAYNOOTH KILDARE IRELAND DEPT AGR NO IRELAND BELFAST BT9 5PX ANTRIM NORTH IRELAND
Titolo Testata:
Journal of nematology
fascicolo: 4, volume: 29, anno: 1997,
pagine: 441 - 450
SICI:
0022-300X(1997)29:4<441:TRITSR>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
FRAGMENT-LENGTH-POLYMORPHISM; RIBOSOMAL DNA-SEQUENCE; MOLECULAR CHARACTERIZATION; PARASITIC NEMATODES; RFLP ANALYSIS; GLOBODERA; DIFFERENTIATION; IDENTIFICATION; CAENORHABDITIS; MELOIDOGYNE;
Keywords:
DIAGNOSIS; GENETICS; INTERNAL TRANSCRIBED SPACER REGION; ITS; MOLECULAR ECOLOGY; NEMATODE; SYSTEMATICS; TAXONOMY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
T.O. Powers et al., "THE RDNA INTERNAL TRANSCRIBED SPACER REGION AS A TAXONOMIC MARKER FORNEMATODES", Journal of nematology, 29(4), 1997, pp. 441-450

Abstract

The ITS region from a wide taxonomic range of nematodes, including secernentean and adenophorean taxa, and free-living, entomopathogenic, and plant-parasitic species, was evaluated as a taxonomic marker. Size of the amplified product aided in the initial determination of group membership, and also suggested groups that may require taxonomic reevaluation. Congeneric species often displayed identically sized ITS regions, but genera such as Pratylenchus and Tylenchorynchus had species with large differences in size. ITS heterogeneity in individuals and populations was identified in several nematode taxa. PCR-RFLP of ITS1 is advocated as a method of taxonomic analysis in genera such as Helicotylenchus that contain numerous species with few diagnostic morphological characteristics.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 05:40:32