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Titolo:
EXCITOTOXIC SWELLING OCCURS IN OXYGEN AND GLUCOSE DEPRIVED HUMAN CORTICAL SLICES
Autore:
WERTH JL; PARK TS; SILBERGELD DL; ROTHMAN SM;
Indirizzi:
ST LOUIS CHILDRENS HOSP,DEPT NEUROL,1 CHILDRENS PL ST LOUIS MO 63110 WASHINGTON UNIV,SCH MED,CTR STUDY NERVOUS SYST INJURY,DEPT NEUROL ST LOUIS MO 63110 WASHINGTON UNIV,SCH MED,EPILEPSY CTR,DEPT NEUROL SURG ST LOUIS MO 63110 ST LOUIS CHILDRENS HOSP,DEPT NEUROSURG ST LOUIS MO 63110 BARNES JEWISH HOSP,DEPT NEUROSURG ST LOUIS MO 63110 ST LOUIS CHILDRENS HOSP,DEPT NEUROL ST LOUIS MO 63110
Titolo Testata:
Brain research
fascicolo: 1-2, volume: 782, anno: 1998,
pagine: 248 - 254
SICI:
0006-8993(1998)782:1-2<248:ESOIOA>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXCITATORY AMINO-ACIDS; GLUTAMATE NEUROTOXICITY; SYNAPTIC TRANSMISSION; HIPPOCAMPAL-NEURONS; RAT HIPPOCAMPUS; BRAIN-SLICES; PHARMACOLOGY; RECEPTORS; HYPOXIA; INVITRO;
Keywords:
CEREBRAL ISCHEMIA; GLUTAMINE; MK-801; NMDA; STROKE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
J.L. Werth et al., "EXCITOTOXIC SWELLING OCCURS IN OXYGEN AND GLUCOSE DEPRIVED HUMAN CORTICAL SLICES", Brain research, 782(1-2), 1998, pp. 248-254

Abstract

The experimental evidence linking glutamate to ischemic neuronal injury is derived from in vitro or in vivo animal stroke models. We, therefore, developed an in vitro preparation to determine whether glutamatecontributes to early neuronal swelling in oxygen and glucose deprived(OGD) human neocortical slices. In order to monitor neuronal swelling, we measured extracellular tissue resistance in brain slices by passing constant current pulses through two electrodes and recording the voltage drop between them. We verified that NMDA (30 mu M) or OGD induced a rise in tissue resistance in rat neocortical slices. We then examined human neocortical slices from 11 patients undergoing resections for intractable epilepsy. Both the rodent and human neocortical slices welled within 10 min of OGD. In both, the glutamate antagonist dizocilpine (MK-801) reduced the swelling. In the rats, MK-801 (5 mu M) prolonged the latency to onset of neuronal swelling following OGD from 7.6 +/- 0.6 min (mean +/- S.E.M., n = 16) to 17.4 +/- 2.6 min (n = 6; p < 0.01). Other putative neuroprotective agents were much less effective in this paradigm. In the human slices, MK-801 again prolonged the latency to resistance increase from 8.6 +/- 0.4 min (n = 8) to 17.2 +/- 1.7min (n = 9, p < 0.01). This is the direct demonstration that glutamate receptor activation leads to neuronal swelling in substrate deficient human brain. These results, which are similar to those obtained in the rodent brain slices, help validate the animal slices as appropriatemodels for the study of OGD in human brain. (C) 1998 Elsevier ScienceB.V.

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Documento generato il 30/11/20 alle ore 19:17:49