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Titolo:
ARGINASE ACTIVITY IS MODULATED BY IL-4 AND HOARG IN NEPHRITIC GLOMERULI AND MESANGIAL CELLS
Autore:
WADDINGTON SN; TAM FWK; COOK HT; CATTELL V;
Indirizzi:
IMPERIAL COLL SCH MED ST MARYS,DEPT HISTOPATHOL,NORFOLK PL LONDON W2 1PG ENGLAND IMPERIAL COLL SCH MED ST MARYS,DEPT HISTOPATHOL LONDON W2 1PG ENGLAND HAMMERSMITH HOSP,ROYAL POSTGRAD MED SCH,RENAL UNIT LONDON W12 0NN ENGLAND
Titolo Testata:
American journal of physiology. Renal, fluid and electrolyte physiology
fascicolo: 3, volume: 43, anno: 1998,
pagine: 473 - 480
SICI:
0363-6127(1998)43:3<473:AAIMBI>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
NITRIC-OXIDE SYNTHASE; HYDROXY-L-ARGININE; MARROW-DERIVED MACROPHAGES; GROWTH-FACTOR-BETA; ALVEOLAR MACROPHAGES; RAT; EXPRESSION; GLOMERULONEPHRITIS; INTERLEUKIN-4; INDUCTION;
Keywords:
GLOMERULONEPHRITIS; NITRIC OXIDE; L-ARGININE; N-G-HYDROXY-L-ARGININE; ADENOSINE 3',5'-CYCLIC MONOPHOSPHATE; MACROPHAGES; INTERLEUKIN-4;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
57
Recensione:
Indirizzi per estratti:
Citazione:
S.N. Waddington et al., "ARGINASE ACTIVITY IS MODULATED BY IL-4 AND HOARG IN NEPHRITIC GLOMERULI AND MESANGIAL CELLS", American journal of physiology. Renal, fluid and electrolyte physiology, 43(3), 1998, pp. 473-480

Abstract

Arginase shares a common substrate, L-arginine, with nitric oxide synthase (NOS). Both enzymes are active at inflammatory sites. To understand regulation of arginase and its relationship to nitric oxide (NO) production, we studied effects of N-G-hydroxy-L-arginine (HOArg) and interleukin-4 (IL-4) on urea and NO2- synthesis by glomeruli during rat immune glomerulonephritis and compared these with macrophages and glomerular mesangial cells (MC). In nephritic glomeruli, elicited macrophages, and MC stimulated with IL-1 and adenosine 3',5'-cyclic monophosphate agonists, increased arginase and induced NOS activity was found. Urea production was inhibited by HOArg and increased by IL-4. NO inhibition [N-G-monomethyl-L-arginine (L-NMMA)] increased arginase activity in nephritic glomeruli and macrophages but not MC. NO2- synthesis was inhibited by L-NMMA and IL-4. It was increased with HOArg under conditions of NO inhibition. In contrast, in normal glomeruli and basal MC, where there was no induced NO synthesis, IL-4 had no effect on arginase activity, whereas HOArg consistently reduced it in glomeruli only. Type II arginase (Arg II) mRNA was detected in normal glomeruli; nephritic glomeruli expressed both Arg I and Arg II mRNAs. This is the firstdemonstration of arginase modulation in glomeruli and MC and of the expression of arginase isoforms in glomeruli. The differential responses to two endogenous compounds generated by inflammation suggest this may be part of coordinated regulation of arginase and inducible NOS in immune injury, whereby arginase is inhibited during high-output NO production and stimulated with NO suppression. This, together with control of arginase and NOS isoforms, may be important in controlling the balance of inflammatory and repair mechanisms.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 19:10:15