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Titolo:
WATER AND SOLUTE PERMEABILITIES OF MEDULLARY THICK ASCENDING LIMB APICAL AND BASOLATERAL MEMBRANES
Autore:
RIVERS R; BLANCHARD A; ELADARI D; LEVIEL F; PAILLARD M; PODEVIN RA; ZEIDEL ML;
Indirizzi:
UNIV PITTSBURGH,MED CTR,RENAL ELECTROLYTE DIV,LAB EPITHELIAL CELL BIOL,RM 931 SCAIFE HALL PITTSBURGH PA 15213 UNIV PITTSBURGH,MED CTR,RENAL ELECTROLYTE DIV,LAB EPITHELIAL CELL BIOL PITTSBURGH PA 15213 UNIV PARIS 06,FAC MED,BROUSSAIS HOTEL DIEU,LAB PHYSIOL & ENDOCRINOL CELLULAIRE & MOL PARIS FRANCE INST NATL SANTE & RECH MED U356 PARIS FRANCE
Titolo Testata:
American journal of physiology. Renal, fluid and electrolyte physiology
fascicolo: 3, volume: 43, anno: 1998,
pagine: 453 - 462
SICI:
0363-6127(1998)43:3<453:WASPOM>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
NONELECTROLYTE PERMEABILITIES; INTRACELLULAR PH; COLLECTING DUCT; UREA TRANSPORT; CELL; AMMONIA; PROTEIN; HENLE; ULTRASTRUCTURE; UROPLAKIN;
Keywords:
KIDNEY; MEDULLA; MEDULLARY THICK ASCENDING LIMB; WATER PERMEABILITY; RENAL CONCENTRATING MECHANISM; RENAL AMMONIUM TRANSPORT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
R. Rivers et al., "WATER AND SOLUTE PERMEABILITIES OF MEDULLARY THICK ASCENDING LIMB APICAL AND BASOLATERAL MEMBRANES", American journal of physiology. Renal, fluid and electrolyte physiology, 43(3), 1998, pp. 453-462

Abstract

The medullary thick ascending limb (MTAL) reabsorbs solute without water and concentrates NH4+ in the interstitium without a favorable pH gradient, activities which require low water and NH3 permeabilities. The contributions of different apical and basolateral membrane structures to these low permeabilities are unclear. We isolated highly purifiedapical and basolateral MTAL plasma membranes and measured, by stopped-flow fluorometry, their permeabilities to water, urea, glycerol, protons, and NH3. Osmotic water permeability at 20 degrees C averaged 9.4 /- 0.8 x 10(-4) cm/s for apical and 11.9 +/- 0.5 x 10(-4) cm/s for basolateral membranes. NH3 permeabilities at 20 degrees C averaged 0.0023 +/- 0.00035 and 0.0035 +/- 0.00080 cm/s for apical and basolateral membranes, respectively. These values are consistent with those obtained in isolated perfused tubules and can account for known aspects of MTAL function in vivo. Because the apical and basolateral membrane unit permeabilities are similar, the ability of the apical membrane to function as the site of barrier function arises from its very small surface area when compared with the highly redundant basolateral membrane.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 01:17:34