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Titolo:
ADENOVIRUS-MEDIATED EXPRESSION OF AN ELASTASE-SPECIFIC INHIBITOR (ELAFIN) - A COMPARISON OF DIFFERENT PROMOTERS
Autore:
SALLENAVE JM; XING Z; SIMPSON AJ; GRAHAM FL; GAULDIE J;
Indirizzi:
UNIV EDINBURGH,SCH MED,DEPT MED,RAYNE LAB,TEVIOT PL EDINBURGH EH8 9AGMIDLOTHIAN SCOTLAND MCMASTER UNIV,DEPT PATHOL HAMILTON ON L8S 4L8 CANADA
Titolo Testata:
Gene therapy
fascicolo: 3, volume: 5, anno: 1998,
pagine: 352 - 360
SICI:
0969-7128(1998)5:3<352:AEOAEI>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; MURINE CYTOMEGALO-VIRUS; GENE-THERAPY; RECOMBINANT ADENOVIRUSES; PROTEINASE-INHIBITOR; BRONCHIAL-SECRETIONS; LEUKOCYTE PROTEINASE; SEQUENCE HOMOLOGY; EPITHELIAL-CELLS; HUMAN-LUNG;
Keywords:
ADENOVIRUS; ELASTASE-SPECIFIC INHIBITOR; ELAFIN; PROTEINASE INHIBITOR; GENE THERAPY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
J.M. Sallenave et al., "ADENOVIRUS-MEDIATED EXPRESSION OF AN ELASTASE-SPECIFIC INHIBITOR (ELAFIN) - A COMPARISON OF DIFFERENT PROMOTERS", Gene therapy, 5(3), 1998, pp. 352-360

Abstract

This report describes the design and construction of three recombinant adenoviruses of serotype 5 (Ad5) expressing elafin (EL), also calledelastase-specific inhibitor. Three promoters were chosen to drive thesynthesis of elafin: the small (380 bp) human cytomegalovirus promoter (HCMV), the Ad2 major late promoter (MLP) and the mouse cytomegalovirus (MCMV) promoter. Human alveolar epithelial cells (A549), as well as rat and human primary pulmonary fibroblasts were infected with Ad5-HCMV-EL, Ad5-MLP-EL, Ad5-MCMV-EL and with the control Ad5-dl70/3. The MCMV promoter was the most efficient promoter in ail cells studied. MLPwas the least efficient promoter. Intermediate between MCMV and MLP was HCMV which was able to induce significant amounts of elafin, particularly in human A549 cells. When compared in vivo in rat lungs, results were similar; MCMV was the only promoter which induced significant amounts of elafin as assessed by Northern blot analysis and ELISA, evenwith a low dose of virus (3 x 10(9) p.f.u.). Our data indicate that the MCMV promoter is the promoter of choice for the strong induction ofadenovirus-mediated transgenes in the lung, and suggest its suitability both in rodent experimental models and in humans for investigative and therapeutic purposes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 18:59:43