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Titolo:
INVOLVEMENT OF ANTILIPOARABINOMANNAN ANTIBODIES IN CLASSICAL COMPLEMENT ACTIVATION IN TUBERCULOSIS
Autore:
HETLAND G; WIKER HG; HOGASEN K; HAMASUR B; SVENSON SB; HARBOE M;
Indirizzi:
UNIV OSLO,NATL HOSP,INST IMMUNOL & RHEUMATOL,FR QVAMSGT 1 N-0172 OSLONORWAY UPPSALA UNIV,DEPT VET MICROBIOL,SECT BACTERIOL UPPSALA SWEDEN
Titolo Testata:
Clinical and diagnostic laboratory immunology
fascicolo: 2, volume: 5, anno: 1998,
pagine: 211 - 218
SICI:
1071-412X(1998)5:2<211:IOAAIC>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
MYCOBACTERIUM-BOVIS BCG; LINKED IMMUNOSORBENT-ASSAY; MANNAN-BINDING-PROTEIN; HUMAN MACROPHAGES; NONOPSONIC BINDING; HUMAN MONOCYTES; SERUM; LIPOARABINOMANNAN; PHAGOCYTOSIS; RECEPTORS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
G. Hetland et al., "INVOLVEMENT OF ANTILIPOARABINOMANNAN ANTIBODIES IN CLASSICAL COMPLEMENT ACTIVATION IN TUBERCULOSIS", Clinical and diagnostic laboratory immunology, 5(2), 1998, pp. 211-218

Abstract

We examined alternative and classical complement activation induced by whole bacilli of Mycobacterium bovis BCG and Mycobacterium tuberculosis products. After exposure to BCG, there were higher levels of the terminal complement complex in sera from Indian tuberculosis patients than in sera from healthy controls. The addition of BCG with or withoutEGTA to these sera indicated that approximately 70 to 85% of the total levels of the terminal complement complex was formed by classical activation. Sera from Indian tuberculosis patients contained more antibody to lipoarabinomannan (LAM) than sera from healthy Indians. Levels of anti-LAM immunoglobulin G2 (IgG2), but not anti-LAM IgM, correlated positively with classical activation induced by BCG in the sera. By flow cytometry, deposition of C3 and terminal complement complex on bacilli incubated with normal human serum was demonstrated. The anticomplement staining was significantly reduced in the presence of EGTA and EDTA. Flow cytometry also revealed the binding of complement to BCG incubated with rabbit anti-LAM and then with factor B-depleted serum. Thisindicates that classical activation plays a major role in complement activation induced by mycobacteria and that anti-LAM IgG on the bacilli can mediate this response. Classical complement activation may be important for the extent of phagocytosis of M. tuberculosis by mononuclear phagocytes, which may influence the course after infection.

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Documento generato il 27/09/20 alle ore 01:02:41