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Titolo:
ELECTROPHORETIC APPLICATIONS OF PHYCOBILIPROTEINS
Autore:
ARAOZ R; LEBERT M; HADER DP;
Indirizzi:
UNIV ERLANGEN NURNBERG,LEHRSTUHL BOT 1,INST BOT & PHARMAZEUT BIOL,STAUDTSTR 5 D-90158 ERLANGEN GERMANY UNIV ERLANGEN NURNBERG,LEHRSTUHL BOT 1,INST BOT & PHARMAZEUT BIOL D-90158 ERLANGEN GERMANY
Titolo Testata:
Electrophoresis
fascicolo: 2, volume: 19, anno: 1998,
pagine: 215 - 219
SICI:
0173-0835(1998)19:2<215:EAOP>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHYCOBILISOMES; PROTEINS; CYANOBACTERIA; SUBUNIT;
Keywords:
CYANOBACTERIA; FLUORESCENT PROTEINS; PHYCOCYANIN; PHYCOERYTHRIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
R. Araoz et al., "ELECTROPHORETIC APPLICATIONS OF PHYCOBILIPROTEINS", Electrophoresis, 19(2), 1998, pp. 215-219

Abstract

Phycobiliproteins are homologous chromoproteins which constitute the phycobilisomes, the light harvesting complexes of the photosynthetic apparatus in cyanobacteria, rhodophyta and cryptophyta. In the present work, phycocyanin (PC) and phycoerythrin (PE) from a Nostoc species are proposed as protein markers for electrophoretic techniques. Phycocyanin is a blue-colored phycobiliprotein; it carries phycocyanobilin as chromophoric group and is composed of two subunits, alpha and beta, with M-r of 14000 and 17000, respectively. In contrast, the PE subunits,having a similar M-r of 21000, are deep rose chromoproteins and carryphycoerythrobilin residues. Both low molecular weight phycobiliproteins are also suitable for monitoring protein blotting and the focusing time of protein samples during isoelectric focusing as internal markers. The PE subunits which form a single broad band after sodium dodecylsulfate-polyacrylamide gel electrophoresis have different isoelectricpoints, and they form two visible bands when they reach their isoelectric point. The phycobilisomes constitute up to 50% of the total protein in cyanobacteria and their content in PC or PE can be up-or down-regulated by using different light conditions (chromatic adaptation).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 00:41:02