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Titolo:
CONTROL OF EFFECTIVE EXPRESSION OF THE PHAGE PHI-CTX-ENCODED CTX GENEIN PSEUDOMONAS-AERUGINOSA BY A PROMOTER UPSTREAM OF THE COS SITE
Autore:
XIONG G; LIN J; OEPEN P; SENERWA D; LUTZ F;
Indirizzi:
UNIV GIESSEN,INST PHARMAKOL & TOXIKOL,FRANKFURTER STR 107 D-35392 GIESSEN GERMANY UNIV GIESSEN,INST PHARMAKOL & TOXIKOL D-35392 GIESSEN GERMANY
Titolo Testata:
MGG. Molecular & general genetics
fascicolo: 3, volume: 257, anno: 1998,
pagine: 249 - 254
SICI:
0026-8925(1998)257:3<249:COEEOT>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYTOTOXIN; ATTP; BACTERIOPHAGE; INTEGRATION; REGION;
Keywords:
BACTERIOPHAGE PHI-CTX; CTX GENE; PROMOTER; PSEUDOMONAS AERUGINOSA; SITE-DIRECTED MUTAGENESIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
18
Recensione:
Indirizzi per estratti:
Citazione:
G. Xiong et al., "CONTROL OF EFFECTIVE EXPRESSION OF THE PHAGE PHI-CTX-ENCODED CTX GENEIN PSEUDOMONAS-AERUGINOSA BY A PROMOTER UPSTREAM OF THE COS SITE", MGG. Molecular & general genetics, 257(3), 1998, pp. 249-254

Abstract

In the DNA of bacteriophage phi CTX, the attachment site (attP), the cohesive end site (cos), and the gene (ctx) encoding the pore-forming cytotoxin, are clustered. Deletion variants and PCR-generated fragments of the DNA were cloned into the Pseudomonas aeruginosa and Escherichia coli vector pHA10. Recombinant plasmids carrying the chloramphenicol acetyltransferase gene, cat, were used for promoter studies. Two promoters responsible for ctx expression are located between attP and cosand between cos and ctx, the promoter upstream of cos being stronger than the one downstream. The promoters and the ribosomal binding site are recognized by both the P. aeruginosa and E. coli transcriptional systems. The results indicate that chromosomal integration of phi CTX DNA, which requires cos site ligation, is necessary for high ctx expression in phi CTX-infected P. aeruginosa strains.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 16:23:54