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Titolo:
MECHANISM OF THE LEAKAGE INDUCED ON LIPID MODEL MEMBRANES BY THE HEMOLYTIC PROTEIN STICHOLYSIN-II FROM THE SEA-ANEMONE STICHODACTYLA-HELIANTHUS
Autore:
DELOSRIOS V; MANCHENO JM; LANIO ME; ONADERRA M; GAVILANES JG;
Indirizzi:
UNIV COMPLUTENSE MADRID,FAC QUIM,DEPT BIOQUIM & BIOL MOL E-28040 MADRID SPAIN UNIV COMPLUTENSE MADRID,FAC QUIM,DEPT BIOQUIM & BIOL MOL E-28040 MADRID SPAIN UNIV LA HABANA,FAC BIOL,DEPT BIOQUIM HAVANA CUBA
Titolo Testata:
European journal of biochemistry
fascicolo: 2, volume: 252, anno: 1998,
pagine: 284 - 289
SICI:
0014-2956(1998)252:2<284:MOTLIO>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMINO-ACID-SEQUENCE; ACTINIA-EQUINA L; STOICHACTIS-HELIANTHUS; VESICLE CONTENTS; ENERGY-TRANSFER; ALPHA-SARCIN; CYTOLYSIN; PEPTIDE; TOXIN; SPHINGOMYELIN;
Keywords:
CYTOLYSIN; LIPID VESICLE; MEMBRANE PERMEABILIZATION; PROTEIN-LIPID INTERACTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
V. Delosrios et al., "MECHANISM OF THE LEAKAGE INDUCED ON LIPID MODEL MEMBRANES BY THE HEMOLYTIC PROTEIN STICHOLYSIN-II FROM THE SEA-ANEMONE STICHODACTYLA-HELIANTHUS", European journal of biochemistry, 252(2), 1998, pp. 284-289

Abstract

A potent hemolytic polypeptide, sticholysin II, has been purified to homogeneity from the sea anemone Stichodactyla helianthus. The proteinproduces leakage of aqueous contents of model lipid vesicles composedof either phosphatidylcholine or sphingomyelin if cholesterol is present in these membranes. The leakage has been analyzed by measuring thedequenching of the fluorescent dye 8-aminonaphthalene-1,3,6-trisulfonic acid, coencapsulated with its quencher N,N'-p-xylenebispyridinium bromide, upon dilution of the vesicle contents into the external medium. The protein displays a maximum effect on vesicles containing. 20-25%cholesterol. Leakage is also produced in vesicles composed of mixtures of phosphatidylcholine and sphingomyelin, the maximum effect being observed for 20-30% sphingomyelin molar content. The extent of the leakage is dependent on the molecular moss of the vesicle entrapped solutes in the range 445-960 Da. This suggests the involvement of a pore of about 1 nm in diameter based on the limiting size observed for the leakage of the different solutes. Oligomerization of the protein is apparently involved in the membrane permeabilization, based on the kinetic analysis of the leakage process which is shown to proceed through an all-or-none mechanism.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 23:28:58