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Titolo:
REGULATION AND FUNCTIONAL-CHARACTERIZATION OF A RAT RECOMBINANT DOPAMINE D3 RECEPTOR
Autore:
COX BA; ROSSER MP; KOZLOWSKI MR; DUWE KM; NEVE RL; NEVE KA;
Indirizzi:
DEPT VET AFFAIRS MED CTR,MED RES SERV 151LL,3710 SW US VET HOSP RED PORTLAND OR 97201 DEPT VET AFFAIRS MED CTR,MED RES SERV 151LL PORTLAND OR 97201 OREGON HLTH SCI UNIV,DEPT PHARMACOL & PSYCHIAT PORTLAND OR 97201 OREGON HLTH SCI UNIV,DEPT BIOCHEM & MOLEC BIOL PORTLAND OR 97201 BRISTOL MYERS SQUIBB CO,PHARMACEUT RES INST,DEPT SCREENING & BIOCHEM RES WALLINGFORD CT 06492 HARVARD UNIV,SCH MED,DEPT GENET BELMONT MA 02178 HARVARD UNIV,SCH MED,DEPT PSYCHIAT BELMONT MA 02178 MCLEAN HOSP BELMONT MA 02178
Titolo Testata:
Synapse
fascicolo: 1, volume: 21, anno: 1995,
pagine: 1 - 9
SICI:
0887-4476(1995)21:1<1:RAFOAR>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
NA-H EXCHANGE; ADENYLATE-CYCLASE; I-125 EPIDEPRIDE; PITUITARY-CELLS; EXTRACELLULAR ACIDIFICATION; D2-DOPAMINE RECEPTORS; LACTOTROPH CELLS; GENE-TRANSFER; D2 RECEPTORS; LIVING CELLS;
Keywords:
CDNA; ANTIPORTER; SIGNAL TRANSDUCTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
56
Recensione:
Indirizzi per estratti:
Citazione:
B.A. Cox et al., "REGULATION AND FUNCTIONAL-CHARACTERIZATION OF A RAT RECOMBINANT DOPAMINE D3 RECEPTOR", Synapse, 21(1), 1995, pp. 1-9

Abstract

We stably expressed a rat D3 receptor cDNA in C-6 glioma cells (C6-D3cells), quantifying receptor expression with the radioligands [I-125]epidepride (K-D = 0.1 nM) and [H-3]spiperone (K-D = 0.7 nM). As reported previously for D2 receptors, quinpirole induced a 9-16% increase inthe rate of extracellular acidification by C6-D3 cells. The acidification was inhibited by epidepride and by the Na+/H+ antiporter inhibitors, amiloride and methylisobutylamiloride, but pertussis toxin treatment had no effect on quinpirole-induced extracellular acidification. These data suggest that D3 receptor stimulation of Na+/H+ exchange in C-6 glioma cells is not mediated by the pertussis toxin-sensitive G proteins, G(i) or G(o). Overnight treatment of C6-D3 cells with N-propylnorapomorphine, dopamine, or quinpirole resulted in large concentration-dependent increases (up to 500%) in the density of D3 receptors on membranes prepared from the cells. Antagonists had smaller, variable effects on the density of D3 receptors in C6-D3 cells, except for domperidone, which significantly increased the density of D3 receptors. Treatment with pertussis toxin had no effect on the agonist-induced receptorup-regulation, indicating that an interaction with pertussis toxin-sensitive G proteins was not required. Densitometry analysis of Northernblots of RNA prepared from C6-D3 cells showed no significant N-propylnorapomorphine-induced increase in D3 receptor message. Treatment withcycloheximide, however, completely prevented receptor up-regulation by N-propylnorapomorphine. Pretreatment of C6-D2 cells with 10 mu M DA resulted in a substantial heterologous sensitization, in which isoproterenol-stimulated adenylyl cyclase activity was enhanced more than twofold. In contrast, isoproterenol-stimulated enzyme activity was inhibited by greater than 50% in C6-D3 cells pretreated with dopamine. Theseresults confirm one functional response to activation of D3 receptorsand demonstrate that the density of D3 receptors, like D2 receptors, is increased after incubation of intact cells with agonists. (C) 1995 Wiley-Liss, Inc.()

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/11/19 alle ore 03:08:53