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Titolo:
DIFFERENCES IN REVERSE-TRANSCRIPTASE ACTIVITY VERSUS P24 ANTIGEN-DETECTION IN CELL-CULTURE, WHEN COMPARING A HOMOGENEOUS GROUP OF HIV TYPE-1 SUBTYPE-B VIRUSES WITH A HETEROGENEOUS GROUP OF DIVERGENT STRAINS
Autore:
CORRIGAN GE; ALKHALILI L; MALMSTEN A; THORSTENSSON R; FENYO EM; KALLANDER CFR; GRONOWITZ JS;
Indirizzi:
CAVIDI TECH AB,UPPSALA SCI PK S-75183 UPPSALA SWEDEN KAROLINSKA INST,MTC S-17177 STOCKHOLM SWEDEN UNIV UPPSALA,DEPT MED GENET,RES UNIT REPLICAT ENZYMOL S-75124 UPPSALASWEDEN SWEDISH INST INFECT DIS CONTROL S-10521 STOCKHOLM SWEDEN
Titolo Testata:
AIDS research and human retroviruses
fascicolo: 4, volume: 14, anno: 1998,
pagine: 347 - 352
SICI:
0889-2229(1998)14:4<347:DIRAVP>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNODEFICIENCY; INFECTIONS; CAMEROON;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
22
Recensione:
Indirizzi per estratti:
Citazione:
G.E. Corrigan et al., "DIFFERENCES IN REVERSE-TRANSCRIPTASE ACTIVITY VERSUS P24 ANTIGEN-DETECTION IN CELL-CULTURE, WHEN COMPARING A HOMOGENEOUS GROUP OF HIV TYPE-1 SUBTYPE-B VIRUSES WITH A HETEROGENEOUS GROUP OF DIVERGENT STRAINS", AIDS research and human retroviruses, 14(4), 1998, pp. 347-352

Abstract

Failure to detect infection with HIV-1 non-B subtypes in some antibody screening assays has been shown, To date, however, no studies have been published evaluating the capacity of standard tests to quantify replication of divergent HIV-1 in cell culture, Reverse transcriptase (RT) activity and p24 antigen assays are the two methods most commonly used for this purpose, A homogeneous panel of HIV-1 subtype B viruses from northern Italy and a heterogeneous panel of diverse genetic subtypes (A to F and 0) from different regions of the world were cultured under identical conditions, A new nonradioactive RT assay was used as a basis for comparison to evaluate the capacity of two p24 assays to quantify viral growth in both panels. Comparison of the p24 amount/RT activity (p24/RT) ratios showed that ratios in the subtype B panel tendedto be markedly higher than in the diverse subtype panel, Greatest variation was seen with one of the subtype O isolates, where up to a 400 times lower ratio was obtained compared with the average ratio for thesubtype B panel, In addition, one Thai subtype B virus also gave a markedly reduced ratio, Furthermore, comparison between the two p24 assays showed different abilities to detect p24 from different HIV-1 isolates, We discuss limitations for the use of anti-HIV-1 p24 antibodies produced by immunization with subtype B p24 in p24 assays.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 13:45:27