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Titolo:
TRANSDUCTION OF RECOMBINANT-HUMAN-ERYTHROPOIETIN RECEPTOR CDNA INTO DAUGHTER PROGENITORS DERIVED FROM SINGLE CD34(3-BLOOD CELLS CHANGES THEDIFFERENTIATION PROFILE OF DAUGHTER PROGENITORS() CORD)
Autore:
LU L; LI ZH; HE J; GE Y; RICE S; BROXMEYER HE;
Indirizzi:
INDIANA UNIV,SCH MED,WALTHER ONCOL CTR,1044 W WALNUT ST,ROOM 302 INDIANAPOLIS IN 46202 INDIANA UNIV,SCH MED,DEPT MICROBIOL & IMMUNOL INDIANAPOLIS IN 46202 INDIANA UNIV,SCH MED,DEPT MED HEMATOL ONCOL INDIANAPOLIS IN 46202 WALTHER CANC INST INDIANAPOLIS IN 00000
Titolo Testata:
Journal of leukocyte biology
fascicolo: 3, volume: 63, anno: 1998,
pagine: 389 - 394
SICI:
0741-5400(1998)63:3<389:TORRCI>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEDIATED GENE TRANSDUCTION; ACTIVATED ERYTHROPOIETIN; COLONY FORMATION; BONE-MARROW; EXPRESSION; CULTURE; SYP;
Keywords:
VIRAL VECTORS; ERYTHROID DIFFERENTIATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
L. Lu et al., "TRANSDUCTION OF RECOMBINANT-HUMAN-ERYTHROPOIETIN RECEPTOR CDNA INTO DAUGHTER PROGENITORS DERIVED FROM SINGLE CD34(3-BLOOD CELLS CHANGES THEDIFFERENTIATION PROFILE OF DAUGHTER PROGENITORS() CORD)", Journal of leukocyte biology, 63(3), 1998, pp. 389-394

Abstract

In this study, we tested the capacity to change the differentiation profile of progenitor cells by retroviral-mediated transduction of EpoRcDNA into one of the paired daughter cells derived from single CD34(3) CB cells, Our results show that for the non-viral-treated daughter cells, the majority (99.6%) formed the same colony type. However, withcells transduced with viral vectors, 7.1% of the daughter cells transduced with the EpoR cDNA formed either a burst forming unit-erythroid (BFU-E) or a colony-forming unit-granulocyte, macrophage, erythroid, megakaryocyte (CFU-GEMM) colony compared to the other daughter cell. transduced with viral supernatant lacking EpoR cDNA, which formed eithera colony-forming unit granulocyte-macrophage (CFU-GM) or a high proliferative potential-colony forming cell (HPP-CFC) colony, Expression ofthe transduced EpoR cDNA was confirmed iu individual colonies by RT-PCR analysis, These results substantiate in a more rigorous fashion ourprevious results that it is possible to change the Epo-responsive differentiation profile of progenitor cells by transduction into these cells of an EpoR cDNA and this change was apparent only in daughter cells derived from single CD34(3+) kit(+) cells transduced with EpoR cDNA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 18:01:50