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Titolo:
A SUBTRACTIVE HYBRIDIZATION METHOD FOR THE ENRICHMENT OF MODERATELY INDUCED SEQUENCES
Autore:
KONIETZKO U; KUHL D;
Indirizzi:
UNIV HAMBURG,ZENTRUM MOL NEUROBIOL ZMNH,MARTINISTR 52 D-20246 HAMBURGGERMANY UNIV HAMBURG,ZENTRUM MOL NEUROBIOL ZMNH D-20246 HAMBURG GERMANY
Titolo Testata:
Nucleic acids research
fascicolo: 5, volume: 26, anno: 1998,
pagine: 1359 - 1361
SICI:
0305-1048(1998)26:5<1359:ASHMFT>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
DIFFERENTIALLY EXPRESSED GENES; IMMEDIATE-EARLY GENE; MESSENGER-RNA; SYNAPTIC ACTIVITY; HYBRIDIZATION; DISPLAY; CDNA; SEIZURE; NEURONS; CLONING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
22
Recensione:
Indirizzi per estratti:
Citazione:
U. Konietzko e D. Kuhl, "A SUBTRACTIVE HYBRIDIZATION METHOD FOR THE ENRICHMENT OF MODERATELY INDUCED SEQUENCES", Nucleic acids research, 26(5), 1998, pp. 1359-1361

Abstract

Moderately induced genes often escape detection in conventional subtraction hybridisation cloning. Here a modification of a phagemid subtraction protocol is described that overcomes this problem, The protocol uses low ratio hybridisation of driver to target sequences to allow enrichment of the sequences of interest, and back-hybridisation of the subtracted sequences with induced sequences to reduce the accumulation of false positive clones. The procedure takes advantage of the quantitative representation of cellular RNA populations in cDNA libraries, therefore, they may serve not only as renewable sources of driver and target sequences, but also as sources of population cRNAs used in northern blots and differential Southern blots.

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Documento generato il 15/07/20 alle ore 14:07:33