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Titolo:
HSP70 ACCUMULATION IN CHONDROCYTIC CELLS EXPOSED TO HIGH CONTINUOUS HYDROSTATIC-PRESSURE COINCIDES WITH MESSENGER-RNA STABILIZATION RATHER THAN TRANSCRIPTIONAL ACTIVATION
Autore:
KAARNIRANTA K; ELO M; SIRONEN R; LAMMI MJ; GOLDRING MB; ERIKSSON JE; SISTONEN L; HELMINEN HJ;
Indirizzi:
UNIV KUOPIO,DEPT ANAT,POB 1627 FIN-70211 KUOPIO FINLAND UNIV KUOPIO,DEPT ANAT FIN-70211 KUOPIO FINLAND UNIV TURKU,ABO AKAD UNIV,TURKU CTR BIOTECHNOL TURKU 20521 FINLAND HARVARD UNIV,SCH MED BOSTON MA 02115 BETH ISRAEL DEACONESS MED CTR BOSTON MA 02115
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 5, volume: 95, anno: 1998,
pagine: 2319 - 2324
SICI:
0027-8424(1998)95:5<2319:HAICCE>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEAT-SHOCK FACTOR; ARTICULAR-CARTILAGE CHONDROCYTES; MESSENGER-RNA; GENE-TRANSCRIPTION; 3'-UNTRANSLATED REGION; PROTEOGLYCAN SYNTHESIS; BIOSYNTHETIC RESPONSE; CULTURED CHONDROCYTES; CHICKEN RETICULOCYTES; DYNAMIC COMPRESSION;
Keywords:
HEAT SHOCK TRANSCRIPTION FACTOR; HEAT SHOCK ELEMENT; CARTILAGE; CHONDROCYTE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
58
Recensione:
Indirizzi per estratti:
Citazione:
K. Kaarniranta et al., "HSP70 ACCUMULATION IN CHONDROCYTIC CELLS EXPOSED TO HIGH CONTINUOUS HYDROSTATIC-PRESSURE COINCIDES WITH MESSENGER-RNA STABILIZATION RATHER THAN TRANSCRIPTIONAL ACTIVATION", Proceedings of the National Academy of Sciences of the United Statesof America, 95(5), 1998, pp. 2319-2324

Abstract

In response to various stress stimuli, heat shock genes are induced to express heat shock proteins (Hsps), Previous studies have revealed that expression of heat shock genes is regulated both at transcriptional and posttranscriptional level, and the rapid transcriptional induction of heat shock genes involves activation of the specific transcription factor, heat shock factor 1 (HSF1). Furthermore, the transcriptional induction can vary in intensity and kinetics in a signal-and cell-type-dependent manner. In this study, we demonstrate that mechanical loading in the form of hydrostatic pressure increases heat shock gene expression in human chondrocyte-like cells. The response to continuous high hydrostatic pressure was characterized by elevated mRNA and proteinlevels of Hsp70, without activation of HSF1 and transcriptional induction of hsp70 gene, The increased expression of Hsp70 was mediated through stabilization of hsp70 mRNA molecules, Interestingly, in contrastto static pressurization, cyclic hydrostatic loading did not result in the induction of heat shock genes, Our findings show that hsp70 geneexpression is regulated posttranscriptionally without transcriptionalinduction in chondrocyte-like cells upon exposure to high continuous hydrostatic pressure, We suggest that the posttranscriptional regulation in the form of hsp70 mRNA stabilization provides an additional modeof heat shock gene regulation that is likely to be of significant importance in certain forms of stress.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 12:08:32