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Titolo:
THE USE OF HETEROLOGOUS PROMOTERS FOR ADENOASSOCIATED VIRUS (AAV) PROTEIN EXPRESSION IN AAV VECTOR PRODUCTION
Autore:
OGASAWARA Y; URABE M; OZAWA K;
Indirizzi:
JICHI MED SCH,INST HEMATOL,DEPT MOL BIOL,3311-1 YAKUSHIJI MINAMI KAWACHI TOCHIGI 3290431 JAPAN JICHI MED SCH,INST HEMATOL,DEPT MOL BIOL MINAMI KAWACHI TOCHIGI 3290431 JAPAN JIKEI UNIV,SCH MED,DEPT INTERNAL MED 2 TOKYO 1050003 JAPAN
Titolo Testata:
Microbiology and immunology
fascicolo: 3, volume: 42, anno: 1998,
pagine: 177 - 185
SICI:
0385-5600(1998)42:3<177:TUOHPF>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADENOASSOCIATED VIRUS; GENE-THERAPY; REP PROTEINS; INTEGRATION; MUTANTS; REPLICATION; PARVOVIRUS; CELLS; SITE; DNA;
Keywords:
AAV VECTOR; HELPER PLASMID; HETEROLOGOUS PROMOTER; GENE THERAPY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
Y. Ogasawara et al., "THE USE OF HETEROLOGOUS PROMOTERS FOR ADENOASSOCIATED VIRUS (AAV) PROTEIN EXPRESSION IN AAV VECTOR PRODUCTION", Microbiology and immunology, 42(3), 1998, pp. 177-185

Abstract

Although adeno-associated virus (AAV) vectors are potentially useful gene transfer vehicles for gene therapy, the vector production system is currently at the developmental stage. We constructed AAV helper plasmids (Rep and Cap expression plasmids) by replacing a native AAV promoter, p5, with various heterologous promoters to examine whether the efficiency of AAV vector production was influenced by modulating the AAV protein expression pattern. The helper plasmids containing heterologous promoters (EF, CMV, SV40, B19p6, and CAG promoters, respectively) expressed Rep78/68 more efficiently than a conventional helper plasmid(pIM45), but the expression of Rep52/40 and Cap decreased, resulting in a significant reduction in AAV vector production. Furthermore, the efficiency of vector production never fully recovered even if the Cap proteins were supplied by an additional expression plasmid. A large amount of Rep78/68 and/or a reduced level of Rep52/40 may have deleterious effects on AAV vector production. The present findings will aid in the development of a more efficient AAV vector production system.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/05/20 alle ore 01:00:48