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Titolo:
REGULATION OF THE ASSOCIATION OF ADDUCIN WITH ACTIN-FILAMENTS BY RHO-ASSOCIATED KINASE (RHO-KINASE) AND MYOSIN PHOSPHATASE
Autore:
KIMURA K; FUKATA Y; MATSUOKA Y; BENNETT V; MATSUURA Y; OKAWA K; IWAMATSU A; KAIBUCHI K;
Indirizzi:
NARA INST SCI & TECHNOL,DIV SIGNAL TRANSDUCT,8916-5 TAKAYAMA IKOMA 63001 JAPAN NARA INST SCI & TECHNOL,DIV SIGNAL TRANSDUCT IKOMA 63001 JAPAN KYOTO UNIV,GRAD SCH MED,DEPT ANAT & NEUROBIOL,SAKYO KU KYOTO 606 JAPAN DUKE UNIV,MED CTR,HOWARD HUGHES MED INST DURHAM NC 27710 DUKE UNIV,MED CTR,DEPT CELL BIOL DURHAM NC 27710 DUKE UNIV,MED CTR,DEPT BIOCHEM DURHAM NC 27710 NATL INST INFECT DIS,DEPT VIROL 2,SHINJYUKU KU TOKYO 162 JAPAN KIRIN BREWERY CO LTD,CENT LABS KEY TECHNOL,KANAZAWA KU YOKOHAMA KANAGAWA 236 JAPAN
Titolo Testata:
The Journal of biological chemistry
fascicolo: 10, volume: 273, anno: 1998,
pagine: 5542 - 5548
SICI:
0021-9258(1998)273:10<5542:ROTAOA>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
GTP-BINDING-PROTEIN; SERINE-THREONINE KINASE; SMOOTH-MUSCLE; ERYTHROCYTE ADDUCIN; PUTATIVE TARGET; MEMBRANE; PHOSPHORYLATION; FAMILY; INVOLVEMENT; ACTIVATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
52
Recensione:
Indirizzi per estratti:
Citazione:
K. Kimura et al., "REGULATION OF THE ASSOCIATION OF ADDUCIN WITH ACTIN-FILAMENTS BY RHO-ASSOCIATED KINASE (RHO-KINASE) AND MYOSIN PHOSPHATASE", The Journal of biological chemistry, 273(10), 1998, pp. 5542-5548

Abstract

The small GTPase Rho is believed to regulate the actin cytoskeleton and cell adhesion through its specific targets. We previously identified the Rho targets: protein kinase N, Rho-associated kinase (Rho-kinase), and the myosin-binding subunit (MBS) of myosin phosphatase. Here wepurified MBS-interacting proteins, identified them as adducin, and found that MBS specifically interacted with adducin in vitro and in vivo. Adducin is a membrane-skeletal protein that promotes the binding of spectrin to actin filaments and is concentrated at the cell-cell contact sites in epithelial cells. We also found that Rho-kinase phosphorylated alpha-adducin in vitro and in vivo and that the phosphorylation of alpha-adducin by Rho-kinase enhanced the interaction of alpha-adducin with actin filaments in vitro. Myosin phosphatase composed of the catalytic subunit and MBS showed phosphatase activity toward alpha-adducin, which was phosphorylated by Rho-kinase. This phosphatase activity was inhibited by the phosphorylation of MBS by RHO-kinase. These results suggest that Rho-kinase and myosin phosphatase regulate the phosphorylation state of adducin downstream of Rho and that the increased phosphorylation of adducin by Rho-kinase causes the interaction of adducin with actin filaments.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/21 alle ore 13:38:56