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Titolo:
TELOMERIC DNA OF BOTRYTIS-CINEREA - A USEFUL TOOL FOR STRAIN IDENTIFICATION
Autore:
LEVIS C; GIRAUD T; DUTERTRE M; FORTINI D; BRYGOO Y;
Indirizzi:
INRA,PATHOL VEGETALE STN,ROUTE ST CYR F-78026 VERSAILLES FRANCE
Titolo Testata:
FEMS microbiology letters
fascicolo: 2, volume: 157, anno: 1997,
pagine: 267 - 272
SICI:
0378-1097(1997)157:2<267:TDOB-A>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
ELECTROPHORETIC KARYOTYPE; FILAMENTOUS FUNGI; TRANSFORMATION; POLYMORPHISM; GENE;
Keywords:
FINGERPRINT; TELOMERE; BOTRYTIS CINEREA; FILAMENTOUS FUNGUS;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
C. Levis et al., "TELOMERIC DNA OF BOTRYTIS-CINEREA - A USEFUL TOOL FOR STRAIN IDENTIFICATION", FEMS microbiology letters, 157(2), 1997, pp. 267-272

Abstract

Telomeric DNA was isolated from the phytopathogenic fungus Botrytis cinerea by PCR using only the oligonucleotide primer (CCCTAA)(4). As with other filamentous fungi, B. cinerea has a short TTAGGG telomeric repeat. Telomere-linked restriction fragment length polymorphism (RFLP) was found in strains of B. cinerea isolated from different host plantscollected from different regions at different periods. Almost every strain had a specific RFLP pattern, including those collected from the same plant one month apart. Thus, this marker appears to be an excellent tool to show the great polymorphism of B. cinerea strains by fingerprinting. The Southern blots of some strains of B. cinerea showed one band which was much more intense than the others, suggesting that the majority of telomere-associated sequences have the same sequence.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 12:47:06