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Titolo:
CAMPYLOBACTER-JEJUNI MAJOR OUTER-MEMBRANE PROTEIN AND A 59-KDA PROTEIN ARE INVOLVED IN BINDING TO FIBRONECTIN AND INT 407 CELL-MEMBRANES
Autore:
MOSER I; SCHROEDER W; SALNIKOW J;
Indirizzi:
FREE UNIV BERLIN,INST MIKROBIOL & TIERSEUCHEN,FABECKSTR 36A D-14195 BERLIN GERMANY FREE UNIV BERLIN,INST BIOCHEM D-14195 BERLIN GERMANY TECH UNIV BERLIN,INST BIOCHEM D-10587 BERLIN GERMANY
Titolo Testata:
FEMS microbiology letters
fascicolo: 2, volume: 157, anno: 1997,
pagine: 233 - 238
SICI:
0378-1097(1997)157:2<233:CMOPAA>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
SURFACE-PROTEINS; INVITRO BINDING; INFECTION; IDENTIFICATION; COLI;
Keywords:
CAMPYLOBACTER; MAJOR OUTER MEMBRANE PROTEIN; 59-KDA OUTER MEMBRANE PROTEIN; ADHESION; INT 407 CELL; FIBRONECTIN;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
I. Moser et al., "CAMPYLOBACTER-JEJUNI MAJOR OUTER-MEMBRANE PROTEIN AND A 59-KDA PROTEIN ARE INVOLVED IN BINDING TO FIBRONECTIN AND INT 407 CELL-MEMBRANES", FEMS microbiology letters, 157(2), 1997, pp. 233-238

Abstract

Campylobacter jejuni is one of the major causes of human diarrhea throughout the world. Attachment to host cells and extracellular matrix proteins is considered to be an essential primary event in the pathogenesis of enteritis. Outer membrane proteins of three C. jejuni strains,one of which was aflagellate, were investigated for their contribution to the process of adhesion to INT 407 cell membranes and the extracellular matrix protein fibronectin. Using a ligand-binding immunoblotting assay the flagellin, the major outer membrane protein and a 59-kDa protein were detected to be involved in adhesion to both substrates. The MOMP was able to inhibit the attachment of the bacteria to INT 407 cell membranes partly, when the protein was isolated under native conditions. However, it was totally lost when the protein was isolated in the presence of SDS. The 59-kDa protein of one strain was identified by N-terminal sequencing, and regarding the first 14 amino acids it wasfound to be identical to the 37-kDa CadF protein just recently described as fibronectin-binding protein of C. jejuni. Especially for the aflagellate strain this protein may be of special importance for adhesion of the bacteria to different substrates.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 21:25:56