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Titolo:
CLONING AND ANALYSIS OF THE GLUCOSYL TRANSFERASE GENE ENCODING TYPE-IANTIGEN IN SHIGELLA-FLEXNERI
Autore:
BASTIN DA; LORD A; VERMA NK;
Indirizzi:
AUSTRALIAN NATL UNIV,FAC SCI,SCH LIFE SCI,DEPT BIOCHEM & MOL BIOL CANBERRA ACT 0200 AUSTRALIA AUSTRALIAN NATL UNIV,FAC SCI,SCH LIFE SCI,DEPT BIOCHEM & MOL BIOL CANBERRA ACT 0200 AUSTRALIA
Titolo Testata:
FEMS microbiology letters
fascicolo: 1, volume: 156, anno: 1997,
pagine: 133 - 139
SICI:
0378-1097(1997)156:1<133:CAAOTG>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
O-ANTIGEN; IMMUNOGENICITY; BANGLADESH; DISEASE; VACCINE; REGION; SFL124; STRAIN; SFX;
Keywords:
SHIGELLA FLEXNERI; GLUCOSYL TRANSFERASE GENE;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
D.A. Bastin et al., "CLONING AND ANALYSIS OF THE GLUCOSYL TRANSFERASE GENE ENCODING TYPE-IANTIGEN IN SHIGELLA-FLEXNERI", FEMS microbiology letters, 156(1), 1997, pp. 133-139

Abstract

The O-antigen of most Shigella flexneri serotypes contains an identical tetrasaccharide repeating unit. Apart from serotype Y, the O-antigen is modified by addition of a glucosyl and/or O-acetyl residue to a specific position in the O-unit. In this study the glucosyl transferasegene from a serotype 1a has been cloned and identified. The bacteriophage SfV integrase (int) gene was used to probe a S. flexneri Y53 (serotype 1a) cosmid library and 18 unique clones were identified. Southern hybridisation of these clones indicated two unlinked regions of the chromosome contained the int homologue. When expressed in a live candidate vaccine strain of S. flexneri serotype Y (SFL124), clones with one region produced type I antigen, whereas clones containing the other region produced mainly type Y antigen. One of the cosmid clones positive for type I antigen by agglutination and Western blotting was selected for further study. Genes involved in O-antigen glucosyl modification were mapped on a 5.8 kb fragment and subclones were produced which fully or partially expressed the type I antigen, depending on the extent of the clone. Fully and partially expressing clones may be useful vaccine candidate strains for protection against disease caused by two serotypes of S. flexneri.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 05:47:55