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Titolo:
DIFFERENTIATION OF ACINETOBACTER-BAUMANNII BIOTYPES BY AMPLIFICATION OF 16S-23S RIBOSOMAL-RNA INTERGENIC SPACER SEQUENCES
Autore:
GARCIA A; MONTOYA R; BELLO H; GONZALEZ G; DOMINGUEZ M; ZEMELMAN R;
Indirizzi:
CONCEPCION UNIV,FAC CIENCIAS BIOL,DEPT MICROBIOL CONCEPCION CHILE CONCEPCION UNIV,FAC CIENCIAS BIOL,DEPT MOL BIOL CONCEPCION CHILE
Titolo Testata:
Microbios
fascicolo: 356, volume: 88, anno: 1996,
pagine: 159 - 167
SICI:
0026-2633(1996)88:356<159:DOABBA>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMORPHIC DNA ANALYSIS; GENUS ACINETOBACTER; IDENTIFICATION; CALCOACETICUS; EPIDEMIOLOGY; PCR; HAEMOLYTICUS; STRAINS;
Keywords:
ACINETOBACTER BAUMANNII; BIOTYPES; GENETIC DISCRIMINATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
A. Garcia et al., "DIFFERENTIATION OF ACINETOBACTER-BAUMANNII BIOTYPES BY AMPLIFICATION OF 16S-23S RIBOSOMAL-RNA INTERGENIC SPACER SEQUENCES", Microbios, 88(356), 1996, pp. 159-167

Abstract

Isolates of Acinetobacter baumannii (32 strains) from blood samples obtained from patients in five Chilean hospitals were identified and biotyped according to their phenotypic properties. They were also submitted to random amplified polymorphic DNA (RAPD) using eight randomly designed 10-mers and the core sequence of M13 phage (15-mers) as well asamplification of the spacer regions between 16S and 23S genes in the prokaryotic rRNA genetic loci. With some primers, RAPD discriminated between biotypes, whereas with others each isolate showed a particular profile. When amplification of spacer regions was performed, a clear correlation between patterns and biotypes was found. This last technique allowed correct biotyping of clinical isolates. Both genetic methodsmight be used for the identification of A. baumannii biotypes.

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Documento generato il 27/10/20 alle ore 04:53:42