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Titolo:
BINDING-CAPACITY OF FK506 BINDING-PROTEIN AFTER 2-HOUR HEMISPHERIC ISCHEMIA IN GERBIL BRAIN
Autore:
NOZAKI H; TANAKA K; SHIRAI T; NAGATA E; KONDO T; KOYAMA S; DEMBO T; FUKUUCHI Y;
Indirizzi:
KEIO UNIV,SCH MED,DEPT NEUROL,SHINJUKU KU,35 SHINANOMACHI TOKYO 160 JAPAN
Titolo Testata:
Brain research
fascicolo: 1-2, volume: 781, anno: 1998,
pagine: 1 - 7
SICI:
0006-8993(1998)781:1-2<1:BOFBA2>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
CEREBRAL BLOOD-FLOW; AUTORADIOGRAPHIC ANALYSIS; 2ND-MESSENGER SYSTEMS; NERVOUS-SYSTEM; IMMUNOSUPPRESSANT; IMMUNOPHILINS; CALCINEURIN;
Keywords:
FK506; FK506 BINDING PROTEIN; CALCINEURIN; CEREBRAL ISCHEMIA; CEREBRAL BLOOD FLOW; GERBIL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
H. Nozaki et al., "BINDING-CAPACITY OF FK506 BINDING-PROTEIN AFTER 2-HOUR HEMISPHERIC ISCHEMIA IN GERBIL BRAIN", Brain research, 781(1-2), 1998, pp. 1-7

Abstract

The binding capacity of FK506 binding protein (FKBP) was examined after 2-h hemispheric ischemia in the gerbil brain in order to clarify the precise mechanism of the neuroprotective effects of FK506. Firstly, the FK506 binding was evaluated in vitro in the normal gerbil brain using 1 nM [H-3]dihydro-FK506 as a specific ligand. FK506 binding sites were distributed in a rather homogeneous manner, although the greatestbinding was noted in the hippocampus CA1. Secondly, Scatchard analysis demonstrated that the binding sites of FK506 could be composed of two components in each brain region. Thirdly, 18 Mongolian gerbils were divided into two groups: an ischemia group (n = 12) and a sham group (n = 6). The right common carotid artery was ligated to induce hemispheric ischemia for 2 h in the ischemia group. The local cerebral blood flow was measured at the end of the experiment by the [C-14]iodoantipyrine method. The ligated animals with levels of local cerebral blood flow in the lateral nuclei of the thalamus of less than 50 ml/100 g/min were utilized as the ischemia group (n = 6) for further data analysis. No significant differences in FK506 binding between the ischemia and sham groups were observed in any regions. The above data indicate thatthe binding capacity of FKBP tends to remain normal during 2-h ischemia, suggesting that FK506 may exert its neuroprotective effects through its binding to FKBP in the brain during the early phase of cerebral ischemia. (C) 1998 Elsevier Science B.V.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 07:06:02