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Titolo:
DIRECT MASS-SPECTROMETRIC PEPTIDE PROFILING AND SEQUENCING OF SINGLE NEURONS REVEALS DIFFERENTIAL PEPTIDE PATTERNS IN A SMALL NEURONAL NETWORK
Autore:
JIMENEZ CR; LI KW; DREISEWERD K; SPIJKER S; KINGSTON R; BATEMAN RH; BURLINGAME AL; SMIT AB; VANMINNEN J; GERAERTS WPM;
Indirizzi:
FREE UNIV AMSTERDAM,FAC BIOL,DEPT MOL & CELLULAR NEUROBIOL,GRAD SCH NEUROSCI AMSTERDAM NL-1081 HV AMSTERDAM NETHERLANDS FREE UNIV AMSTERDAM,FAC BIOL,DEPT MOL & CELLULAR NEUROBIOL,GRAD SCH NEUROSCI AMSTERDAM NL-1081 HV AMSTERDAM NETHERLANDS MICROMASS MANCHESTER M23 9LZ LANCS ENGLAND UNIV CALIF SAN FRANCISCO,MASS SPECTROMETR FACIL,DEPT PHARMACEUT CHEM SAN FRANCISCO CA 94143
Titolo Testata:
Biochemistry
fascicolo: 7, volume: 37, anno: 1998,
pagine: 2070 - 2076
SICI:
0006-2960(1998)37:7<2070:DMPPAS>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
SNAIL LYMNAEA-STAGNALIS; CARDIOACTIVE PEPTIDES; NEUROPEPTIDES; APLYSIA; EXPRESSION; GENE; RPD2; VD1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
C.R. Jimenez et al., "DIRECT MASS-SPECTROMETRIC PEPTIDE PROFILING AND SEQUENCING OF SINGLE NEURONS REVEALS DIFFERENTIAL PEPTIDE PATTERNS IN A SMALL NEURONAL NETWORK", Biochemistry, 37(7), 1998, pp. 2070-2076

Abstract

Mass spectrometry (MS) was employed to detect and structurally characterize peptides in two functionally related neurons, named VT)I and RPD2, which form a network involved in the modulation of heartbeat in Lymnaea. Matrix-assisted laser desorption/ionization MSI directly applied to single neurons VDI and RPD2, showed overlapping yet distinct massprofiles, with a subset of putative peptides specifically present in neuron VD1. Direct tandem MS of a single VD neuron revealed the primary structures of the VD1-specific peptides, which were identified as members of the family of small cardioactive peptides. Based on the tandem MS data, a degenerate oligonucleotide was made for use in a polymerase chain reaction strategy to isolate the cDNA encoding the precursor to the small cardioactive peptides from a brain-specific cDNA library,The calculated masses of the mature, posttranslationally modified peptides, as predicted from the corresponding cDNA, agreed with the measured masses of the actual peptides, as detected in single-cell MS analysis, In situ hybridization studies showed that the transcript encodingthe precursor is present in VD1, but not in RPD2, thus corroborating the single-cell MS analysis, Finally, the small cardioactive peptides were shown to enhance the contractions of the auricle in vitro.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 20:31:24