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Titolo:
EVIDENCE FOR IN-VIVO RIBOSOME RECYCLING, THE 4TH STEP IN PROTEIN-BIOSYNTHESIS
Autore:
JANOSI L; MOTTAGUITABAR S; ISAKSSON LA; SEKINE Y; OHTSUBO E; ZHANG S; GOON S; NELKEN S; SHUDA M; KAJI A;
Indirizzi:
UNIV PENN,SCH MED,DEPT MICROBIOL PHILADELPHIA PA 19104 UNIV PENN,SCH MED,DEPT MICROBIOL PHILADELPHIA PA 19104 STOCKHOLM UNIV,DEPT MICROBIOL S-10691 STOCKHOLM SWEDEN UNIV TOKYO,INST MOL & CELLULAR BIOSCI TOKYO JAPAN
Titolo Testata:
EMBO journal
fascicolo: 4, volume: 17, anno: 1998,
pagine: 1141 - 1151
SICI:
0261-4189(1998)17:4<1141:EFIRRT>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
ELONGATION-FACTOR-G; ESCHERICHIA-COLI; TRANSLATION TERMINATION; MODULATION FACTOR; NASCENT PEPTIDE; CODON CONTEXT; AMINO-ACID; GENE; SEQUENCE; MUTATIONS;
Keywords:
RIBOSOMAL FRAMESHIFT; RIBOSOME RECYCLING; TEMPERATURE-SENSITIVE RRF; TERMINATION COMPLEX; TRANSLATIONAL RE-INITIATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
L. Janosi et al., "EVIDENCE FOR IN-VIVO RIBOSOME RECYCLING, THE 4TH STEP IN PROTEIN-BIOSYNTHESIS", EMBO journal, 17(4), 1998, pp. 1141-1151

Abstract

Ribosome recycling factor (RRF) catalyzes the fourth step of protein synthesis in vitro: disassembly of the post-termination complex of ribosomes, mRNA and tRNA. We now report the first in vivo evidence of RRFfunction using 12 temperature-sensitive Escherichia coli mutants which we isolated in this study. At non-permissive temperatures, most of the ribosomes remain on mRNA, scan downstream from the termination codon, and re-initiate translation at various sites in all frames without the presence of an initiation codon. Reinitiation does not occur upstream from the termination codon nor beyond a downstream initiation signal. RRF inactivation was bacteriostatic in the growing phase and bactericidal during the transition between the stationary and growing phase, confirming the essential nature of the fourth step of protein synthesis in vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 14:40:21