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Titolo:
RAPID PROTEIN IDENTIFICATION USING A MICROSCALE ELECTROSPRAY LC MS SYSTEM ON AN ION-TRAP MASS-SPECTROMETER/
Autore:
DAVIS MT; LEE TD;
Indirizzi:
CITY HOPE NATL MED CTR,BECKMAN RES INST,DIV IMMUNOL,1450 E DUARTE RD DUARTE CA 91010 CITY HOPE NATL MED CTR,BECKMAN RES INST,DIV IMMUNOL DUARTE CA 91010
Titolo Testata:
Journal of the American Society for Mass Spectrometry
fascicolo: 3, volume: 9, anno: 1998,
pagine: 194 - 201
SICI:
1044-0305(1998)9:3<194:RPIUAM>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEQUENCE DATABASES; POLYACRYLAMIDE GELS; PEPTIDE MIXTURES; FRAGMENTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
M.T. Davis e T.D. Lee, "RAPID PROTEIN IDENTIFICATION USING A MICROSCALE ELECTROSPRAY LC MS SYSTEM ON AN ION-TRAP MASS-SPECTROMETER/", Journal of the American Society for Mass Spectrometry, 9(3), 1998, pp. 194-201

Abstract

A methodology has been developed for the rapid identification of gel separated proteins. Following in gel protein digestion with trypsin, the resulting peptide mixture is analyzed by on-line liquid chromatography, electrospray mass spectrometry (LC/MS). The mass spectral data containing either accurate mass values or sequence specific fragment ioninformation is then matched to a database of known protein sequences. Key features of the LC/MS system are the use of a novel integrated, microscale LC column-electrospray interface and variable flow solvent delivery to optimize the efficiency of sample loading and gradient elution. With these enhancements, only 10 min is required to analyze each sample. The method is routine for sample amounts ranging from 50 to 500 fmol. The analysis parameters for the ion trap mass spectrometer have to be carefully adjusted in order to keep pace with the rapidly eluting LC peaks. Although designed for rapid LC separations, the integrated column-electrospray interface is also able to provide extended analyses of selected components using a technique known as ''peak parking. '' (C) 1998 American Society for Mass Spectrometry.

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Documento generato il 25/10/20 alle ore 12:27:03