Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
DIFFERENTIAL REGULATION OF GONADOTROPIN-RELEASING-HORMONE (GNRH) RECEPTOR EXPRESSION IN THE POSTERIOR MEDIOBASAL HYPOTHALAMUS BY STEROID-HORMONES - IMPLICATION OF GNRH NEURONAL-ACTIVITY
Autore:
SEONG JY; KANG SS; KAM K; HAN YG; KWON HB; RYU K; KIM K;
Indirizzi:
SEOUL NATL UNIV,COLL NAT SCI,DEPT MOL BIOL SEOUL 151742 SOUTH KOREA SEOUL NATL UNIV,COLL NAT SCI,DEPT MOL BIOL SEOUL 151742 SOUTH KOREA SEOUL NATL UNIV,COLL NAT SCI,RES CTR CELL DIFFERENTIAT SEOUL 151742 SOUTH KOREA CHONNAM NATL UNIV,DEPT BIOL KWANGJU 500757 SOUTH KOREA CHONNAM NATL UNIV,HORMONE RES CTR KWANGJU 500757 SOUTH KOREA YONSEI UNIV,COLL MED,DEPT PHARMACOL SEOUL 120749 SOUTH KOREA
Titolo Testata:
Molecular brain research
fascicolo: 1-2, volume: 53, anno: 1998,
pagine: 226 - 235
SICI:
0169-328X(1998)53:1-2<226:DROG(R>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT ESTROUS-CYCLE; MESSENGER-RNA LEVELS; GENE-EXPRESSION; RIBONUCLEIC-ACID; OVARIECTOMIZED RATS; FEMALE RATS; PROGESTERONE; PITUITARY; ESTRADIOL; SURGE;
Keywords:
GONADOTROPIN-RELEASING HORMONE; GONADOTROPIN-RELEASING HORMONE RECEPTOR; ESTROGEN; PROGESTERONE; GENE EXPRESSION; ANTISENSE OLIGONUCLEOTIDE; PREOPTIC AREA; MEDIOBASAL HYPOTHALAMUS; RAT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
J.Y. Seong et al., "DIFFERENTIAL REGULATION OF GONADOTROPIN-RELEASING-HORMONE (GNRH) RECEPTOR EXPRESSION IN THE POSTERIOR MEDIOBASAL HYPOTHALAMUS BY STEROID-HORMONES - IMPLICATION OF GNRH NEURONAL-ACTIVITY", Molecular brain research, 53(1-2), 1998, pp. 226-235

Abstract

The present study is designed to evaluate the relationship between gonadotropin-releasing hormone (GnRH) and GnRH receptor (GnRHR) gene expression during the steroid-induced LH surge. One week after ovariectomy (OVX), a capsule containing 17 beta-estradiol (E) or vehicle (V) wasimplanted into OVX rats, and 2 days later a single injection of progesterone (P) or V was administered s.c. at 10:00 h. Poly(A)-rich RNA samples were isolated from the micropunches of the preoptic area (POA) and the posterior mediobasal hypothalamus (pMBH) from both sides of individual brain slices. Using competitive reverse transcription-polymerase chain reaction (RT-PCR) procedures, three parameters (POA GnRH, pMBH GnRHR and pituitary GnRHR mRNA levels) were simultaneously determined in each individual animal. POA GnRH mRNA and pituitary GnRHR mRNA levels were decreased by treatment with E, but increased by a combination of E and P. In contrast, pMBH GnRHR mRNA levels were clearly augmented by treatment with E, and decreased by the combination of E and P. Temporal changes in such parameters were determined in OVX + E + V-and OVX + E + P-treated rats. P augmented POA GnRH mRNA levels at the timeof the LH surge (17:00 h) and the increased GnRH mRNA levels were remained until 22:00 h, while E alone failed to alter POA GnRH mRNA levels. In the pMBH micropunch samples, P substantially decreased E-inducedincrease in GnRHR mRNA levels at 17:00 h and further lowered those until 22:00 h. Antisense oligonucleotides of GnRHR mRNA administered into the lateral ventricle of OVX + E-treated rats. blocked the E-inducedincrease in pMBH GnRHR mRNA levels. The antisense oligonucleotides also prevented the LH surge as well as the increase in pituitary GnRHR mRNA levels in the OVX + E + P-treated group. However, administration of this antisense oligonucleotides failed to alter POA GnRH mRNA levels. In conclusion, the present study demonstrated that there is an inverse relationship between POA GnRH mRNA levels and pMBH GnRHR mRNA levels in response to E and/or P, and that the blockade of the E-induced increase in pMBH GnRHR mRNA levels effectively nullified the P-induced LH surge. These results indicate that pMBH GnRHR gene expression is involved in synchronizing the GnRH neuronal activity, which is crucial for the generation of the LH surge. (C) 1998 Elsevier Science B.V.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 00:04:47