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Titolo:
REGULATION OF SEROTONIN REUPTAKE TRANSPORTER MESSENGER-RNA EXPRESSIONBY OVARIAN-STEROIDS IN RHESUS MACAQUES
Autore:
PECINSTHOMPSON M; BROWN NA; BETHEA CL;
Indirizzi:
OREGON REG PRIMATE RES CTR,DIV REPROD SCI,505 NW 185TH AVE BEAVERTON OR 97006 OREGON REG PRIMATE RES CTR,DIV REPROD SCI BEAVERTON OR 97006 OREGON REG PRIMATE RES CTR,DIV NEUROSCI BEAVERTON OR 97006 OREGON HLTH SCI UNIV,DEPT PHYSIOL PORTLAND OR 97201
Titolo Testata:
Molecular brain research
fascicolo: 1-2, volume: 53, anno: 1998,
pagine: 120 - 129
SICI:
0169-328X(1998)53:1-2<120:ROSRTM>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROLACTIN-RELEASE; PROGESTIN RECEPTORS; MESSENGER-RNA; PREMENSTRUAL-SYNDROME; MONKEY HYPOTHALAMUS; IMIPRAMINE BINDING; DEPRESSED-PATIENTS; MENOPAUSAL WOMEN; HEALTHY CONTROLS; TREATED MONKEYS;
Keywords:
ESTROGEN; PROGESTERONE; DORSAL RAPHE; SEROTONIN; SERT; PRIMATE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
62
Recensione:
Indirizzi per estratti:
Citazione:
M. Pecinsthompson et al., "REGULATION OF SEROTONIN REUPTAKE TRANSPORTER MESSENGER-RNA EXPRESSIONBY OVARIAN-STEROIDS IN RHESUS MACAQUES", Molecular brain research, 53(1-2), 1998, pp. 120-129

Abstract

It has been widely hypothesized that the ovarian steroids, estrogen (E) and progesterone (P), act on serotonin neurons to modulate mood andincrease prolactin secretion in women. However, information is neededon the molecular consequences of ovarian hormone action in serotonin neurons. This study examined the effect of E and P on the expression of mRNA for the serotonin re-uptake transporter (SERT) in monkeys usingin situ hybridization and a 253 bp human SERT cRNA probe. Monkeys (n = 5 animals/group) were ovariectomized and hysterectomized (spayed) and then untreated (control), or treated with E for 28 days (E treated),or treated with E for 28 days and supplemented with P for the last 14days of the E regimen (E + P treated). Densitometric analysis of autoradiographs with gray-level thresholding was performed at five levels of the dorsal and median raphe. The number of pixels exceeding background in defined areas was obtained (pixel number). The average pixel number for spayed, E- and E + P-treated groups was 22280 +/- 3517, 15 227 +/- 1714, and 14 827 +/- 2042, respectively, in the combined dorsal and median raphe. In the E- and E + P-treated groups compared to the control group, there was a 32% and 33% decrease in SERT mRNA signal represented by pixel number (ANOVA, P < 0.05). Hence, E- and E + P-treated groups were significantly less than the control group, but they werenot different from one another, Also, there were significantly fewer SERT mRNA-positive cells in the dorsal raphe of E- and E + P-treated groups (ANOVA, P < 0.001). Therefore E, with or without P, reduces SERTmRNA expression. These results suggest that the ability of P to increase prolactin secretion in E-primed monkeys does not involve an actionat the level of SERT gene transcription. Hence, the mechanism by which the CNS transduces the action of P on prolactin secretion remains tobe elucidated. However, these data suggest that one action of E replacement therapy in postmenopausal women may be to decrease expression of the SERT gene. (C) 1998 Elsevier Science B.V.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 21:45:55