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Titolo:
ELEVATION OF D-4 DOPAMINE-RECEPTOR MESSENGER-RNA IN POSTMORTEM SCHIZOPHRENIC BRAIN
Autore:
STEFANIS NC; BRESNICK JN; KERWIN RW; SCHOFIELD WN; MCALLISTER G;
Indirizzi:
MERCK SHARP & DOHME LTD,RES LABS,NEUROSCI RES CTR,TERLINGS PK,EASTWICK RD HARLOW CM20 2QR ESSEX ENGLAND MERCK SHARP & DOHME LTD,RES LABS,NEUROSCI RES CTR HARLOW CM20 2QR ESSEX ENGLAND INST PSYCHIAT LONDON SE5 8AF ENGLAND
Titolo Testata:
Molecular brain research
fascicolo: 1-2, volume: 53, anno: 1998,
pagine: 112 - 119
SICI:
0169-328X(1998)53:1-2<112:EODDMI>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
CEREBRAL-CORTEX; D4 RECEPTORS; PREFRONTAL CORTEX; GENE-EXPRESSION; NEURONS; BINDING; LOCALIZATION; HALOPERIDOL; CLONING; CELLS;
Keywords:
HUMAN D-4 DOPAMINE RECEPTOR; SCHIZOPHRENIA; RIBONUCLEASE PROTECTION ASSAY; FRONTAL CORTEX; CAUDATE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
N.C. Stefanis et al., "ELEVATION OF D-4 DOPAMINE-RECEPTOR MESSENGER-RNA IN POSTMORTEM SCHIZOPHRENIC BRAIN", Molecular brain research, 53(1-2), 1998, pp. 112-119

Abstract

The D-4 dopamine (DA) receptor has been proposed to be a target for the development of a novel antipsychotic drug based on its pharmacological and distribution profile. There is much interest in whether D-4 DAreceptor levels are altered in schizophrenia, but the lack of an available receptor subtype-specific radioligand made this difficult to quantitate. In this study, we examined whether D-4 mRNA levels are altered in different brain regions of schizophrenics compared to controls. Ribonuclease protection assays were carried out on total RNA samples isolated postmortem from frontal cortex and caudate brain regions of schizophrenics and matched controls. P-32-labelled RNA probes to the D-4 DA receptor and to the housekeeping gene, glyceraldehyde-3-phosphate dehydrogenase (G3PDH), were hybridised with the RNA samples, digested with ribonucleases to remove unhybridised probe, and separated on 6% sequencing gels. Densitometer analysis on the subsequent autoradiogams was used to calculate the relative optical density of D-4 mRNA comparedto G3PDH mRNA. Statistical analysis of the data revealed a 3-fold higher level (P < 0.011) of D-4 mRNA in the frontal cortex of schizophrenics compared to controls. No increase was seen in caudate. D-4 receptors could play a role in mediating dopaminergic activity in frontal cortex, an activity which may be malfunctioning in schizophrenia. (C) 1998 Elsevier Science B.V.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 17/01/20 alle ore 20:17:35