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Titolo:
IDENTIFICATION OF THE SUBUNITS OF THE NICOTINIC CHOLINERGIC RECEPTORSIN THE RAT COCHLEA USING RT-PCR AND IN-SITU HYBRIDIZATION
Autore:
MORLEY BJ; LI HS; HIEL H; DRESCHER DG; ELGOYHEN AB;
Indirizzi:
BOYS TOWN NATL RES HOSP,NEUROCHEM LAB,555 N 30TH ST OMAHA NE 68131 NIDCD,NEUROCHEM LAB,NIH BETHESDA MD 00000 WAYNE STATE UNIV,DEPT OTOLARYNGOL & BIOCHEM,LAB BIOOTOL DETROIT MI 00000 CONSEJO NACL INVEST CIENT & TECN,INST INVEST FARMACOL RA-1033 BUENOS AIRES DF ARGENTINA
Titolo Testata:
Molecular brain research
fascicolo: 1-2, volume: 53, anno: 1998,
pagine: 78 - 87
SICI:
0169-328X(1998)53:1-2<78:IOTSOT>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
OUTER HAIR-CELLS; ACETYLCHOLINE-RECEPTOR; GENE FAMILY; GUINEA-PIG; INNERVATION; EXPRESSION; MECHANISM; ALPHA-5; SYSTEM; INNER;
Keywords:
MESSENGER-RNA; GENE EXPRESSION; COCHLEA; HAIR CELLS; SPIRAL GANGLION; NICOTINIC ACETYLCHOLINE RECEPTOR SUBUNITS; IN SITU HYBRIDIZATION; RT-PCR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
B.J. Morley et al., "IDENTIFICATION OF THE SUBUNITS OF THE NICOTINIC CHOLINERGIC RECEPTORSIN THE RAT COCHLEA USING RT-PCR AND IN-SITU HYBRIDIZATION", Molecular brain research, 53(1-2), 1998, pp. 78-87

Abstract

There are two tissues in the adult mammalian cochlea that are post-synaptic to cholinergic efferent fibers: The outer hair cells (OHCs) andthe dendrites of the afferent fibers of the type I spiral ganglion cells. The unusual nicotinic-like pharmacology of cochlear cholinergic responses and the unique embryonic development of cochlear tissues suggest that the inner-ear nicotinic cholinergic receptor (nAChR) may be different from nAChRs described previously at synapses in the mammalianbrain, autonomic ganglia, or skeletal muscle. In this study, we determined the mRNA expression of the alpha 2-7, alpha 9, and beta 2-4 subunits of the nicotinic acetylcholine receptor (nAChR) family in the ratcochlea. In micro-dissected tissue from the organ of Corti, spiral ganglion, and the membranous lateral wall, we found mRNA expression of the alpha 7 and alpha 9 subunits in the organ of Corti and alpha 5-7, and beta 2 and beta 3 in the spiral ganglion using RT-PCR. Employing insitu hybridization with S-35-riboprobes, we localized alpha 9 in haircells regions and alpha 6, alpha 7 and beta 2 in the type I cells of the spiral ganglion. No evidence of nAChR subunit mRNA expression was found in supporting cells, but beta 2 was expressed in type II spiral ganglion cells, which are neither cholinergic nor cholinoceptive. (C) 1998 Elsevier Science B.V.

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Documento generato il 09/07/20 alle ore 23:16:19