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Titolo:
CHROMOSOME STABILITY IS MAINTAINED BY SHORT INTERCENTROMERIC DISTANCEIN FUNCTIONALLY DICENTRIC HUMAN ROBERTSONIAN TRANSLOCATIONS
Autore:
PAGE SL; SHAFFER LG;
Indirizzi:
BAYLOR COLL MED,DEPT MOL & HUMAN GENET HOUSTON TX 77030 BAYLOR COLL MED,DEPT MOL & HUMAN GENET HOUSTON TX 77030
Titolo Testata:
Chromosome research
fascicolo: 2, volume: 6, anno: 1998,
pagine: 115 - 122
SICI:
0967-3849(1998)6:2<115:CSIMBS>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
ALPHA-SATELLITE DNA; PROTEIN CENP-C; FLUORESCENCE INSITU HYBRIDIZATION; CENTROMERE PROTEIN; SACCHAROMYCES-CEREVISIAE; ACROCENTRIC CHROMOSOMES; INACTIVE CENTROMERES; DOWNS-SYNDROME; CHAT SYNDROME; SEQUENCE;
Keywords:
CENTROMERE; CENTROMERE PROTEIN C; DICENTRIC CHROMOSOME; ROBERTSONIAN TRANSLOCATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
73
Recensione:
Indirizzi per estratti:
Citazione:
S.L. Page e L.G. Shaffer, "CHROMOSOME STABILITY IS MAINTAINED BY SHORT INTERCENTROMERIC DISTANCEIN FUNCTIONALLY DICENTRIC HUMAN ROBERTSONIAN TRANSLOCATIONS", Chromosome research, 6(2), 1998, pp. 115-122

Abstract

While the formation of a dicentric chromosome often leads to chromosome instability, human dicentric Robertsonian translocations usually remain stable. To investigate the basis for this stability, we have examined the centromeres of 15 structurally dicentric rob(13q14q) Robertsonian translocations using immunofluorescence and fluorescence in situ hybridization (FISH). The immunofluorescence detection of centromere protein C (CENP-C) was used as a marker for centromere function as CENP-C seems to play an essential role in kinetochore structure and stability and was previously shown to be absent from inactive centromeres. In all 15 translocation-containing cell lines, CENP-C was confined to only one of the centromeres of the translocation in a fraction of the cells analyzed. This suggests that centromere inactivation commonly occurs on dicentric Robertsonian translocations and may serve as one mechanism allowing for their stability. However, in the majority of the translocations (12 out of 15), a portion of the cells analyzed displayedCENP-C immunofluorescence at both centromeres, suggesting that both centromeres were active and that the translocation was functionally dicentric. The percentage of cells with CENP-C at both centromeres rangedfrom 2% to 82%. These results support the hypothesis that the close proximity of two functional centromeres on Robertsonian translocations allows them to remain stable.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 09:49:13