Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
5-HT AUTORECEPTORS IN THE REGULATION OF 5-HT RELEASE FROM GUINEA-PIG RAPHE NUCLEUS AND HYPOTHALAMUS
Autore:
MORET C; BRILEY M;
Indirizzi:
PIERRE FABRE RES CTR,17 AVE JEAN MOULIN F-81100 CASTRES FRANCE
Titolo Testata:
Neuropharmacology
fascicolo: 11-12, volume: 36, anno: 1997,
pagine: 1713 - 1723
SICI:
0028-3908(1997)36:11-12<1713:5AITRO>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN CEREBRAL-CORTEX; RECOMBINANT HUMAN 5-HT1D-ALPHA; 5-HT1D-BETA RECEPTOR SUBTYPES; BRAIN CORTEX; IN-VITRO; 5-HYDROXYTRYPTAMINE RELEASE; FUNCTIONAL-CHARACTERIZATION; SEROTONIN AUTORECEPTORS; SPECIES-DIFFERENCES; RAT HYPOTHALAMUS;
Keywords:
GUINEA PIG; 5-HT AUTORECEPTORS; RAPHE; HYPOTHALAMUS; RELEASE;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
C. Moret e M. Briley, "5-HT AUTORECEPTORS IN THE REGULATION OF 5-HT RELEASE FROM GUINEA-PIG RAPHE NUCLEUS AND HYPOTHALAMUS", Neuropharmacology, 36(11-12), 1997, pp. 1713-1723

Abstract

5-HT autoreceptors involved in the regulation of 5-HT release in the guinea pig dorsal raphe nucleus have been studied in comparison with those in the hypothalamus. In vitro release was measured in slices of raphe and hypothalamus prelabelled with [H-3]5-HT, superfused with Krebs solution and depolarized electrically. The non-selective 5-HT receptor agonist, 5-carboxamidotryptamine (5-CT) (0.1-10 nM for raphe; 1-100nM for hypothalamus) and antagonist, methiothepin (10-1000 nM), decreased and increased, respectively, the release of [H-3]5-HT evoked by electrical stimulation in either of these regions when given alone. The selective 5-HT1B/D receptor antagonist, GR127935 (100-1000 nM), and the 5-HT1D receptor antagonist, ketanserin (300-1000 nM), had no significant effect on this release in either of these regions. Methiothepin and GR127935 (100-1000 nM) shifted to the right the concentration-effect curve of 5-CT in both the raphe and the hypothalamus. At 300 nM, ketanserin shifted to the right the concentration-effect curve of 5-CT inthe raphe but did not modify the 5-CT curve in the hypothalamus. In microdialysis experiments ketanserin, applied locally at 10 mu M, increased the extracellular levels of 5-HT in the dorsal raphe nucleus of the freely moving guinea pig, whereas 5-HT levels were unchanged in thehypothalamus. Ketanserin at 1 mu M did not affect the decrease in 5-HT output induced by the selective 5-HT1B/D receptor agonist, naratriptan (used at 10 mu M in raphe and 0.1 mu M in hypothalamus), in the raphe or the hypothalamus. In the raphe, WAY100635, a 5-HT1A receptor antagonist, at 1 mu M, did not prevent naratriptan (10 mu M) from reducing the extracellular levels of 5-HT. These results suggest that, in theconditions used in this study, the release of 5-HT in the dorsal raphe nucleus is possibly modulated in part by 5-HT1B receptors but essentially the control is through 5-HT receptors whose subtype is still to be determined. In the hypothalamus, however, it is clear that only 5-HT1B receptors are involved in the modulation of 5-HT neurotransmission. (C) 1998 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 04:39:53