Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
EFFECT OF FREEZING RATES ON THE SURVIVAL OF PENAEID PRAWN LARVAE - A PARAMETER ANALYSIS
Autore:
ARUN R; SUBRAMONIAM T;
Indirizzi:
UNIV MADRAS,DEPT ZOOL,UNIT INVERTEBRATE REPROD,CRYOBIOL DIV MADRAS 600025 TAMIL NADU INDIA UNIV MADRAS,DEPT ZOOL,UNIT INVERTEBRATE REPROD,CRYOBIOL DIV MADRAS 600025 TAMIL NADU INDIA
Titolo Testata:
Cryo-letters
fascicolo: 6, volume: 18, anno: 1997,
pagine: 359 - 368
SICI:
0143-2044(1997)18:6<359:EOFROT>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
CRYOPROTECTANT TOXICITY; EMBRYOS; CRYOPRESERVATION; VITRIFICATION; CRYOBIOLOGY; TOLERANCE;
Keywords:
CRYOPRESERVATION; CRYOPROTECTANT PERMEATION; CRYOPROTECTANT TOXICITY; FREEZING RATE; SHRIMP; PENAEUS MONODON;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
R. Arun e T. Subramoniam, "EFFECT OF FREEZING RATES ON THE SURVIVAL OF PENAEID PRAWN LARVAE - A PARAMETER ANALYSIS", Cryo-letters, 18(6), 1997, pp. 359-368

Abstract

The objective of this paper was to develop a freeze protocol for the cryopreservation of penaeid prawn larvae. A major difficulty in this arises because of incomplete permeation of the cryoprotectant into the system due to a chitinous exoskeleton and a complex body organization. Ethane 1,2 diol and dimethyl sulphoxide were assessed to be the best both in toxicity as well as penetration assays. Combinations of ethanediol and dimethyl sulphoxide were tolerated for more than 80 minutes. Freeze rates below -1 degrees C/min led to mortality due to supercooled fluids before the freezing of the cytoplasm or the external cryoprotectant. Freezing rates between -1 degrees C to -3 degrees C/min caused critical damages within the thermal regimes of -35 degrees C to -45 degrees C. Higher rates between -5 degrees to -30 degrees C/min to final temperatures of -50 degrees to -70 degrees C gave retrieval of 85 to 95% photomotile survivors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 10:38:27